IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells
Li, Y1; Yang, L1; Cui, JT1; Li, WM1; Guo, RF1; Lu, YY1
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2002-04-01
8期:2页:208-212
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]BUCCAL POUCH CARCINOGENESIS ; FUJIAN PROVINCE ; HELICOBACTER-PYLORI ; CHANGLE COUNTY ; CHINA ; S100B ; RISK
英文摘要

AIM: To elucidate molecular mechanism of chemopreventive efficacies of garlic against human gastric cancer (HGC).

METHODS: HGC cell line BGC823 was treated with Allitridi (a kind of garlic extract) and Allitridi-treated and parental BGC823 cDNA libraries were constructed respectively by using lambdaZAP II vector. cDNA Representational Difference Analysis (cDNA RDA) was performed using BamH I cutting-site and abundant cDNA messages provided by the libraries. Northern blot analysis was applied to Identify the obtained difference products.

RESULTS: Two specific cDNA fragments were obtained and characterized to be derived from homo sapiens folate receptoralpha ( FRalpha) gene and calcyclin gene respectively. Northern blot results showed a 4-fold increase In FRalpha gene expression level and 9-told increase In calcyclin mRNA level in BGC823 cells after Allitridi! treatment for 72 h.

CONCLUSION: The method of cDNA RDA based on cDNA libraries combines the high specificity of cDNA RDA with abundant cDNA messages In cDNA library; this expands the application of cDNA library and Increases the specificity of cDNA RDA. Up-regulation of FRalpha gene and calcyclin gene expressions induced by Allitridi provide valuable molecular evidence for the efficacy of garlic in treating HGC as well as other diseases.

语种英语
WOS记录号WOS:000175104600004
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51899
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Beijing Lab Mol Oncol, Beijing Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China
2.First Mil Med Univ, Affiliated Hosp 1, PLA Inst Digest Dis, Dept Gastroenterol, Guangzhou 510000, Guangdong Prov, Peoples R China
3.Neimenggu Med Coll, Dept Physiol, Huhehaote 010000, Neimengguzizhiq, Peoples R China
Recommended Citation
GB/T 7714
Li, Y,Yang, L,Cui, JT,et al. Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(2):208-212.
APA Li, Y,Yang, L,Cui, JT,Li, WM,Guo, RF,&Lu, YY.(2002).Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells.WORLD JOURNAL OF GASTROENTEROLOGY,8(2),208-212.
MLA Li, Y,et al."Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells".WORLD JOURNAL OF GASTROENTEROLOGY 8.2(2002):208-212.
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