学科主题 | 临床医学 |
Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice | |
Sun, W1; Wang, LY1; Zhang, ZM1; Chen, MZ1; Wang, X1 | |
关键词 | Insulin-dependent Diabetes Mellitus Calcitonin Gene-related Peptide Gene Therapy t Helper Lymphocyte |
刊名 | EUROPEAN JOURNAL OF IMMUNOLOGY
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2003 | |
卷 | 33期:1页:233-242 |
收录类别 | SCI |
文章类型 | Article |
WOS标题词 | Science & Technology |
类目[WOS] | Immunology |
研究领域[WOS] | Immunology |
关键词[WOS] | BETA-CELL EXPRESSION ; INTERFERON-GAMMA ; SKELETAL-MUSCLE ; CYTOKINE PRODUCTION ; LIPID-PEROXIDATION ; T-LYMPHOCYTES ; FREE-RADICALS ; PLASMID DNA ; IFN-GAMMA ; NOD MICE |
英文摘要 | The imbalance of Th1/Th2 subsets is an important pathogenic mechanism for insulin-dependent diabetes mellitus (IDDM). Calcitonin gene-related peptide (CGRP) has been found to play important roles in the regulation of T lymphocytes. We hypothesize that exogenous CGRP administration during insulitis may modulate the balance of Th lymphocytes, thereby providing a therapeutic intervention for IDDM. We established CGRIP gene transfer by naked plasmid injection into the skeletal muscles with electroporation enhancement. The effect of CGRP gene transfer on pathogenesis of IDDM was observed in autoimmune diabetic C57BL mice induced by multiple low-dose streptozotocin (MILDS) administration. The treatment significantly decreased morbidity of diabetes, ameliorated hyperglycemia and insulin deficiency, and inhibited lymphocyte infiltration into the islets, indicating the protection of beta cells against autoimmune destruction. CGRP gene transfer significantly inhibited T cell proliferation and secretion of the Th1 cytokine IFN-gamma, increased the level of the Th2 cytokine IL-10, but had no effect on IL-4 and TGF-beta1 secretion. CGRIP gene transfer also decreased IL-12 and IFN-gamma levels in peritoneal effusion. Our results demonstrate that CGRP gene transfer selectively suppresses the pro-inflammatory Th1 subsets and promote anti-inflammatory Th2 subsets, resulting in amelioration of beta cell destruction and reduction of IDDM occurrence in mice with MLDS-induced diabetes. |
语种 | 英语 |
WOS记录号 | WOS:000180623100027 |
Citation statistics | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.bjmu.edu.cn/handle/400002259/51901 |
Collection | 北京大学第三临床医学院_心血管内科 |
作者单位 | 1.Beijing Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China 2.Beijing Univ, Reference Lab, Educ Minist Mol Cardiol, Beijing 100083, Peoples R China |
Recommended Citation GB/T 7714 | Sun, W,Wang, LY,Zhang, ZM,et al. Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2003,33(1):233-242. |
APA | Sun, W,Wang, LY,Zhang, ZM,Chen, MZ,&Wang, X.(2003).Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice.EUROPEAN JOURNAL OF IMMUNOLOGY,33(1),233-242. |
MLA | Sun, W,et al."Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice".EUROPEAN JOURNAL OF IMMUNOLOGY 33.1(2003):233-242. |
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