北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice
作者: Sun, W1; Wang, LY1; Zhang, ZM1; Chen, MZ1; Wang, X1
关键词: insulin-dependent diabetes mellitus ; calcitonin gene-related peptide ; gene therapy ; T helper lymphocyte
刊名: EUROPEAN JOURNAL OF IMMUNOLOGY
发表日期: 2003
卷: 33, 期:1, 页:233-242
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: BETA-CELL EXPRESSION ; INTERFERON-GAMMA ; SKELETAL-MUSCLE ; CYTOKINE PRODUCTION ; LIPID-PEROXIDATION ; T-LYMPHOCYTES ; FREE-RADICALS ; PLASMID DNA ; IFN-GAMMA ; NOD MICE
英文摘要:

The imbalance of Th1/Th2 subsets is an important pathogenic mechanism for insulin-dependent diabetes mellitus (IDDM). Calcitonin gene-related peptide (CGRP) has been found to play important roles in the regulation of T lymphocytes. We hypothesize that exogenous CGRP administration during insulitis may modulate the balance of Th lymphocytes, thereby providing a therapeutic intervention for IDDM. We established CGRIP gene transfer by naked plasmid injection into the skeletal muscles with electroporation enhancement. The effect of CGRP gene transfer on pathogenesis of IDDM was observed in autoimmune diabetic C57BL mice induced by multiple low-dose streptozotocin (MILDS) administration. The treatment significantly decreased morbidity of diabetes, ameliorated hyperglycemia and insulin deficiency, and inhibited lymphocyte infiltration into the islets, indicating the protection of beta cells against autoimmune destruction. CGRP gene transfer significantly inhibited T cell proliferation and secretion of the Th1 cytokine IFN-gamma, increased the level of the Th2 cytokine IL-10, but had no effect on IL-4 and TGF-beta1 secretion. CGRIP gene transfer also decreased IL-12 and IFN-gamma levels in peritoneal effusion. Our results demonstrate that CGRP gene transfer selectively suppresses the pro-inflammatory Th1 subsets and promote anti-inflammatory Th2 subsets, resulting in amelioration of beta cell destruction and reduction of IDDM occurrence in mice with MLDS-induced diabetes.

语种: 英语
WOS记录号: WOS:000180623100027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51901
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
2.Beijing Univ, Reference Lab, Educ Minist Mol Cardiol, Beijing 100083, Peoples R China

Recommended Citation:
Sun, W,Wang, LY,Zhang, ZM,et al. Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2003,33(1):233-242.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, W]'s Articles
[Wang, LY]'s Articles
[Zhang, ZM]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, W]‘s Articles
[Wang, LY]‘s Articles
[Zhang, ZM]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace