IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice
Sun, W1; Wang, LY1; Zhang, ZM1; Chen, MZ1; Wang, X1
关键词Insulin-dependent Diabetes Mellitus Calcitonin Gene-related Peptide Gene Therapy t Helper Lymphocyte
刊名EUROPEAN JOURNAL OF IMMUNOLOGY
2003
33期:1页:233-242
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]BETA-CELL EXPRESSION ; INTERFERON-GAMMA ; SKELETAL-MUSCLE ; CYTOKINE PRODUCTION ; LIPID-PEROXIDATION ; T-LYMPHOCYTES ; FREE-RADICALS ; PLASMID DNA ; IFN-GAMMA ; NOD MICE
英文摘要

The imbalance of Th1/Th2 subsets is an important pathogenic mechanism for insulin-dependent diabetes mellitus (IDDM). Calcitonin gene-related peptide (CGRP) has been found to play important roles in the regulation of T lymphocytes. We hypothesize that exogenous CGRP administration during insulitis may modulate the balance of Th lymphocytes, thereby providing a therapeutic intervention for IDDM. We established CGRIP gene transfer by naked plasmid injection into the skeletal muscles with electroporation enhancement. The effect of CGRP gene transfer on pathogenesis of IDDM was observed in autoimmune diabetic C57BL mice induced by multiple low-dose streptozotocin (MILDS) administration. The treatment significantly decreased morbidity of diabetes, ameliorated hyperglycemia and insulin deficiency, and inhibited lymphocyte infiltration into the islets, indicating the protection of beta cells against autoimmune destruction. CGRP gene transfer significantly inhibited T cell proliferation and secretion of the Th1 cytokine IFN-gamma, increased the level of the Th2 cytokine IL-10, but had no effect on IL-4 and TGF-beta1 secretion. CGRIP gene transfer also decreased IL-12 and IFN-gamma levels in peritoneal effusion. Our results demonstrate that CGRP gene transfer selectively suppresses the pro-inflammatory Th1 subsets and promote anti-inflammatory Th2 subsets, resulting in amelioration of beta cell destruction and reduction of IDDM occurrence in mice with MLDS-induced diabetes.

语种英语
WOS记录号WOS:000180623100027
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51901
专题北京大学第三临床医学院_心血管内科
作者单位1.Beijing Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
2.Beijing Univ, Reference Lab, Educ Minist Mol Cardiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Sun, W,Wang, LY,Zhang, ZM,et al. Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2003,33(1):233-242.
APA Sun, W,Wang, LY,Zhang, ZM,Chen, MZ,&Wang, X.(2003).Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice.EUROPEAN JOURNAL OF IMMUNOLOGY,33(1),233-242.
MLA Sun, W,et al."Intramuscular transfer of naked calcitonin generelated peptide gene prevents autoimmune diabetes induced by multiple low-dose streptozotocin in C57BL mice".EUROPEAN JOURNAL OF IMMUNOLOGY 33.1(2003):233-242.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, W]的文章
[Wang, LY]的文章
[Zhang, ZM]的文章
百度学术
百度学术中相似的文章
[Sun, W]的文章
[Wang, LY]的文章
[Zhang, ZM]的文章
必应学术
必应学术中相似的文章
[Sun, W]的文章
[Wang, LY]的文章
[Zhang, ZM]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。