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学科主题: 药学
题名:
LC Determination of Icariside II in Rat Plasma and Tissues: Application to a Tissue Distribution Study
作者: Wang, Chang-Guang1; Sun, Jia-Bei1; Wang, Jian-Cheng2; Zhang, Qiang2; Zheng, Ying1
关键词: Column liquid chromatography-ultraviolet detection ; Icariside II ; Flavonoids ; Tissue distribution
刊名: CHROMATOGRAPHIA
发表日期: 2011-08-01
DOI: 10.1007/s10337-011-2057-8
卷: 74, 期:3-4, 页:251-258
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: EPIMEDIUM-KOREANUM NAKAI ; IN-VITRO ; LIQUID-CHROMATOGRAPHY ; ICARIIN ; FLAVONOIDS ; CELLS ; DIFFERENTIATION ; GLYCOSIDES ; EXTRACT ; KINASE
英文摘要:

A sensitive and reliable reversed-phase liquid chromatography (RP-LC) with ultraviolet (UV) detection has been developed and validated for the quantification of Icariside II in rat plasma and tissues using Fermononetin as the internal standard. Protein precipitation and liquid-liquid extraction were utilized for plasma and tissue sample preparation, respectively. The analysis was successfully carried out on an Agilent SB-C(18) column (5 mu m, 4.6 x 250 mm) with the implementation of the following conditions: a mobile phase of phosphoric acid solution (0.1%, v/w)-Acetonitrile (55: 45, v/v), a flow rate of 1 mL min(-1), a column temperature of 25 degrees C and a detection wavelength of 270 nm. Good linear relationships of calibration curves were obtained (r(2) > 0.9906) over the investigated concentration range with plasma and tissue samples. The lower limit of quantification (LLOQ) and the limit of detection (LOD) were 0.1 and 0.02 mu g g(-1), respectively (for plasma sample, they were 0.05 and 0.1 mu g mL(-1), respectively). The developed method which was embodied with good precision, accuracy, recovery and stability was corroborated to satisfy the requirements for biomedical sample analysis. This method has been successfully applied to tissue distribution study of Icariside II in rats after a single intravenous dose at 12.5 mg kg(-1). Results suggested that Icariside II was distributed to rat tissues rapidly with greater initial concentrations in kidney, lung and liver. Moderate initial distributions were obtained in rat muscle, heart, bone, spleen and plasma. Low amount of Icariside II was detected in testes, and no Icariside II could be detected in the brain.

语种: 英语
所属项目编号: 008/2007/A1
项目资助者: Macao Science and Technology Development Fund (FDCT Fund)
WOS记录号: WOS:000294068100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51912
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China
2.Peking Univ, Dept Pharmaceut, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Chang-Guang,Sun, Jia-Bei,Wang, Jian-Cheng,et al. LC Determination of Icariside II in Rat Plasma and Tissues: Application to a Tissue Distribution Study[J]. CHROMATOGRAPHIA,2011,74(3-4):251-258.
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