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Platelet-derived growth factor C plays a role in the branchial arch malformations induced by retinoic acid
Han, Jing1; Li, Li1; Zhang, Zhaofeng1; Xiao, Ying1; Lin, Jiuxiang1; Zheng, Liping1; Li, Yong1
关键词Pdgf-c All-trans-retinoic Acid Branchial Arch Morphogenesis Mmp-2 Mmp-14 Timp-2 Craniofacial Development Cell Migration
刊名BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY
2007-03-01
DOI10.1002/bdra.20329
79期:3页:221-230
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Toxicology
研究领域[WOS]Developmental Biology ; Toxicology
关键词[WOS]PROTEASE-ACTIVATED LIGAND ; CRANIAL NEURAL CREST ; PDGF-ALPHA-RECEPTOR ; MATRIX METALLOPROTEINASES ; TISSUE INHIBITOR ; CELL-MIGRATION ; BETA-RECEPTOR ; VITAMIN-A ; EXPRESSION ; MOUSE
英文摘要

BACKGROUND: All-trans-retinoic acid (RA) can produce branchial arch abnormalities in postimplantation rodent embryos cultured in vitro. Platelet-derived growth factor C (PDGF-C) was recently identified as a member of the PDGF ligand family. Many members of the PDGF family are essential for branchial arch morphogenesis and can be regulated by RA. The roles of PDGF-C in branchial arch malformations induced by RA and possible mechanisms were investigated. METHODS: In whole embryo culture (WEC), mouse embryos were exposed to RA at 0, 0.1, 0.4, 1.0, or 10.0 mu M, PDGF-C at 25, 50, or 75 ng/mL, or PDGF-C at 25, 50, or 75 ng/mL containing 0.4 mu M RA. After 48 h of culture, mouse embryos were examined for dysmorphogenesis, and whole-mount immunohistochemistry was applied to PDGF-C. In explant cultures, explants were exposed to the same doses of RA and PDGF-C as WEC. Serniquantitative RT-PCR, zymography, and reverse zymography were used to evaluate the expressions and activities of matrix metalloproteinase (MMP)-2, MMP-14, and tissue inhibitor of metalloproteinase (TIMP)-2. RESULTS: PDGF-C was reduced by RA, and exogenous PDGF-C rescued the branchial arch malformations induced by RA. Moreover, PDGF-C prevented RA-induced inhibition of the migratory ability of mesenchymal cells in the first branchial arch, by regulating the expressions of MMP-2, MMP-14, and TIPM-2. CONCLUSIONS: Our results suggest that RA exposure reduces the expression of PDGF-C. The branchial arch malformations resulting from fetal RA exposure are caused at least partially by loss of PDGF-C and subsequent misregulations of the expressions of MMP-2, MMP-14, and TIMP-2.

语种英语
WOS记录号WOS:000245004400003
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51938
Collection北京大学公共卫生学院
北京大学公共卫生学院_营养与食品卫生学系
北京大学第三临床医学院_胸外科
作者单位1.Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China
2.Peking Univ, Sch Publ Hlth, Dept Food Sci & Nutr, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Han, Jing,Li, Li,Zhang, Zhaofeng,et al. Platelet-derived growth factor C plays a role in the branchial arch malformations induced by retinoic acid[J]. BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY,2007,79(3):221-230.
APA Han, Jing.,Li, Li.,Zhang, Zhaofeng.,Xiao, Ying.,Lin, Jiuxiang.,...&Li, Yong.(2007).Platelet-derived growth factor C plays a role in the branchial arch malformations induced by retinoic acid.BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY,79(3),221-230.
MLA Han, Jing,et al."Platelet-derived growth factor C plays a role in the branchial arch malformations induced by retinoic acid".BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY 79.3(2007):221-230.
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