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A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by H-1 NMR spectroscopy
Zou, Pingping; Tu, Pengfei; Jiang, Yong
刊名ANALYTICAL METHODS
2013
DOI10.1039/c2ay26226f
5期:4页:1062-1067
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]QUINOLINE ALKALOIDS ; NMR ; CHROMATOGRAPHY ; DASYCARPUS ; LIMONOIDS
英文摘要

Dictamni Cortex (Baixianpi) is a commonly used medicinal herb in traditional Chinese medicines (TCMs), possessing various bioactivities. Quinoline alkaloids are regarded as one of the main active components in this herb. In the present study, a more convenient, specific and accurate analytical method using H-1 NMR has been developed for the quantitative determination of dictamnine, one of the main active quinoline alkaloids in Dictamni Cortex. In a less crowded region delta 8.0-8.5, the signal of H-5 of dictamnine was well separated from other signals in chloroform-d(1). The quantity of dictamnine was calculated by means of the integral value of H-5 of dictamnine to that of the known amount of internal standard, pyrazine. This assay was precise and reproducible, with RSDs less than 3%. The recovery results of dictamnine were observed between 92.10 and 108.10% and the limit of detection (LOD) and limit of quantitation (LOQ) were 3.3 mu g ml(-1) and 13.2 mu g ml(-1), respectively. This newly developed method was proven to be highly accurate, precise and robust, for the determination of dictamnine in Dictamni Cortex. The results obtained from H-1 NMR method were also compared with those from conventional HPLC method, and no remarkable difference was found between these two methods.

语种英语
WOS记录号WOS:000314423700035
项目编号2011-1568 ; 81222051 ; 2012ZX09301002-002-002 ; 2012ZX09304-005
资助机构Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ; National Science Fund for Excellent Young Scholars ; National Key Technology R&amp ; D Program "New Drug Innovation" of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51941
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zou, Pingping,Tu, Pengfei,Jiang, Yong. A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by H-1 NMR spectroscopy[J]. ANALYTICAL METHODS,2013,5(4):1062-1067.
APA Zou, Pingping,Tu, Pengfei,&Jiang, Yong.(2013).A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by H-1 NMR spectroscopy.ANALYTICAL METHODS,5(4),1062-1067.
MLA Zou, Pingping,et al."A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by H-1 NMR spectroscopy".ANALYTICAL METHODS 5.4(2013):1062-1067.
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