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学科主题: 药学
题名:
A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by H-1 NMR spectroscopy
作者: Zou, Pingping; Tu, Pengfei; Jiang, Yong
刊名: ANALYTICAL METHODS
发表日期: 2013
DOI: 10.1039/c2ay26226f
卷: 5, 期:4, 页:1062-1067
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]: Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]: QUINOLINE ALKALOIDS ; NMR ; CHROMATOGRAPHY ; DASYCARPUS ; LIMONOIDS
英文摘要:

Dictamni Cortex (Baixianpi) is a commonly used medicinal herb in traditional Chinese medicines (TCMs), possessing various bioactivities. Quinoline alkaloids are regarded as one of the main active components in this herb. In the present study, a more convenient, specific and accurate analytical method using H-1 NMR has been developed for the quantitative determination of dictamnine, one of the main active quinoline alkaloids in Dictamni Cortex. In a less crowded region delta 8.0-8.5, the signal of H-5 of dictamnine was well separated from other signals in chloroform-d(1). The quantity of dictamnine was calculated by means of the integral value of H-5 of dictamnine to that of the known amount of internal standard, pyrazine. This assay was precise and reproducible, with RSDs less than 3%. The recovery results of dictamnine were observed between 92.10 and 108.10% and the limit of detection (LOD) and limit of quantitation (LOQ) were 3.3 mu g ml(-1) and 13.2 mu g ml(-1), respectively. This newly developed method was proven to be highly accurate, precise and robust, for the determination of dictamnine in Dictamni Cortex. The results obtained from H-1 NMR method were also compared with those from conventional HPLC method, and no remarkable difference was found between these two methods.

语种: 英语
所属项目编号: 2011-1568 ; 81222051 ; 2012ZX09301002-002-002 ; 2012ZX09304-005
项目资助者: Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ; National Science Fund for Excellent Young Scholars ; National Key Technology R&amp ; D Program "New Drug Innovation" of China
WOS记录号: WOS:000314423700035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51941
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zou, Pingping,Tu, Pengfei,Jiang, Yong. A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by H-1 NMR spectroscopy[J]. ANALYTICAL METHODS,2013,5(4):1062-1067.
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