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Patterns of T-cell reconstitution by assessment of T-cell receptor excision circle and T-cell receptor clonal repertoire after allogeneic hematopoietic stem cell transplantation in leukemia patients - a study in Chinese patients
Fu, Yue Wen1; Wu, De Pei1; Cen, Jian Nong1; Feng, Yu Feng1; Chang, Wei Rong1; Zhu, Zi Ling1; Qiu, Qiao Chen1; Zhu, Ping1
关键词Allogeneic Hematopoietic Stem Cell Transplantation t Cell Receptor Beta Chain Cdr3 Thymus T-cell Receptor Excision Circles Immune Deficiency Immune Reconstitution Gvhd
刊名EUROPEAN JOURNAL OF HAEMATOLOGY
2007-08-01
DOI10.1111/j.1600-0609.2007.00885.x
79期:2页:138-145
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology
研究领域[WOS]Hematology
关键词[WOS]BONE-MARROW-TRANSPLANTATION ; VERSUS-HOST-DISEASE ; THYMIC FUNCTION ; IMMUNE RECONSTITUTION ; LYMPHOCYTES ; BLOOD ; DIVERSITY ; ADULTS ; IDENTIFICATION ; COMPARTMENT
英文摘要

Objective: Successful allogeneic hematopoietic stem cell transplantation (HSCT) requires reconstitution normal T-cell immunity. Measurement of T-cell receptor excision circles (TRECs) and T-cell receptor beta (TCRBV) CDR3 repertoire is a means of quantifying recent thymic T-cell production and reflecting antigen-specific T-cell clones proliferation. Methods: We used real-time quantitative PCR to detect TRECs from 43 Chinese patients who underwent three kind of allo-HSCT without T-cell depletion. RT-PCR was performed to amplify 24 subfamily genes of TCRBV in 24 patients of them. Results: For haploidentical-D group, the TRECs numbers were lower up to 24 months. For matched-sibling donor (MSD) group, the recovery of TRECs was faster than those of other two groups. TRECs values in matched-unrelated donor (MUD) were in the middle. During 2-19 months after transplantation, there were 6-16 BV subfamilies expressed and 33-48% of them were polyclones. The usage rate of TCRBV and percentage of polyclones in haploidentical-D were less than those of other two groups. Twenty-three CDR3 molecules were obtained from nine patients who were potentially associated with GVHD or CMV infection. Conclusions: Analyzing the changes of TCRBV repertoire and measuring TRECs during immune reconstitution would be useful to determine the host′s current immune status and ability of T-cell immune reconstitution and also to find antigen-specific T-cell clones in the three kinds of HSCT.

语种英语
WOS记录号WOS:000248450800007
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51945
专题北京大学第一临床医学院_血液内科
作者单位1.Soochow Univ, Affiliated Hosp 1, Dept Hematol, Suzhou 215006, Jiangsu, Peoples R China
2.Beijing Univ, Affiliated Hosp 1, Dept Hematol, Beijing 100871, Peoples R China
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GB/T 7714
Fu, Yue Wen,Wu, De Pei,Cen, Jian Nong,et al. Patterns of T-cell reconstitution by assessment of T-cell receptor excision circle and T-cell receptor clonal repertoire after allogeneic hematopoietic stem cell transplantation in leukemia patients - a study in Chinese patients[J]. EUROPEAN JOURNAL OF HAEMATOLOGY,2007,79(2):138-145.
APA Fu, Yue Wen.,Wu, De Pei.,Cen, Jian Nong.,Feng, Yu Feng.,Chang, Wei Rong.,...&Zhu, Ping.(2007).Patterns of T-cell reconstitution by assessment of T-cell receptor excision circle and T-cell receptor clonal repertoire after allogeneic hematopoietic stem cell transplantation in leukemia patients - a study in Chinese patients.EUROPEAN JOURNAL OF HAEMATOLOGY,79(2),138-145.
MLA Fu, Yue Wen,et al."Patterns of T-cell reconstitution by assessment of T-cell receptor excision circle and T-cell receptor clonal repertoire after allogeneic hematopoietic stem cell transplantation in leukemia patients - a study in Chinese patients".EUROPEAN JOURNAL OF HAEMATOLOGY 79.2(2007):138-145.
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