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Preparative isolation and purification of trans-3,5,4 ′-trihydroxystilbene-4 ′-O-beta-D-glucopyranoside and (+)catechin from Rheum tanguticum Maxim. ex Balf. using high-speed counter-current chromatography by stepwise elution and stepwise increasing the flow-rate of the mobile phase
Jin, W; Tu, PF
关键词-trihydroxystilbene-4 &Prime Trans-3 -o-beta-d-glucopyranoside 5 (+)Catechin 4 &Prime High-speed Counter-current Chromatography Rheum Tanguticum
刊名JOURNAL OF CHROMATOGRAPHY A
2005-10-28
DOI10.1016/j.chroma.2005.08.009
1092期:2页:241-245
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SEPARATION
英文摘要

Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of trans-3,5,4 ′-trihydroxystilbene-4 ′-O-beta-D-glucopyranoside (compound 1) and (+)catechin (compound 2) from Rheum tanguticum Maxim. ex Balf. by stepwise elution with a pair of two-phase solvent system composed of ethyl acetate-ethanol-water (25:1:25, v/v) and (5:1:5, v/v), and stepwise increasing the flow-rate of the mobile phase from 0.8 to 2.0 ml min(-1) after 5h. The preparative HSCCC separation was performed on 250mg of crude extract yielding pure compound 1 (10.2mg) and compound 2 (26.7mg) all at purities of over 96% in a single run. The structures of the two compounds have been elucidated by means of spectroscopic methods including MS and H-1, C-13 nuclear magnetic resonance spectroscopy. (c) 2005 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000232510100011
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51947
Collection北京大学药学院_天然药物学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Jin, W,Tu, PF. Preparative isolation and purification of trans-3,5,4 ′-trihydroxystilbene-4 ′-O-beta-D-glucopyranoside and (+)catechin from Rheum tanguticum Maxim. ex Balf. using high-speed counter-current chromatography by stepwise elution and stepwise increasing the flow-rate of the mobile phase[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1092(2):241-245.
APA Jin, W,&Tu, PF.(2005).Preparative isolation and purification of trans-3,5,4 ′-trihydroxystilbene-4 ′-O-beta-D-glucopyranoside and (+)catechin from Rheum tanguticum Maxim. ex Balf. using high-speed counter-current chromatography by stepwise elution and stepwise increasing the flow-rate of the mobile phase.JOURNAL OF CHROMATOGRAPHY A,1092(2),241-245.
MLA Jin, W,et al."Preparative isolation and purification of trans-3,5,4 ′-trihydroxystilbene-4 ′-O-beta-D-glucopyranoside and (+)catechin from Rheum tanguticum Maxim. ex Balf. using high-speed counter-current chromatography by stepwise elution and stepwise increasing the flow-rate of the mobile phase".JOURNAL OF CHROMATOGRAPHY A 1092.2(2005):241-245.
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