学科主题基础医学
Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides
Zou, Xiajuan1; Liu, Dan1; Zhong, Lijun1; Yang, Bin1; Lou, Yaxin1; Yin, Yuxin1,2
关键词Chitosan-gma-apb Glycopeptides Selective Enrichment Nanosphere Maldi-tof-ms
刊名CARBOHYDRATE POLYMERS
2012-10-01
DOI10.1016/j.carbpol.2012.05.090
90期:2页:799-804
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]Chemistry ; Polymer Science
关键词[WOS]MASS-SPECTROMETRY ; LINKED OLIGOSACCHARIDES ; GLYCOPROTEOMICS ; CHROMATOGRAPHY ; PROTEINS
英文摘要

In this study we describe a method for highly specific enrichment of glycopeptides with boronic acid-functionalized chitosan polymeric nanospheres and matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS). This is the first time chitosan has been used to create nanosphere support material for selective enrichment of glycopeptides by modification with glycidyl methacrylate (GMA) and derivatization with 3-aminophenylboronic acid (APB). Due to their multifunctional chemical moieties, these 20-100 nm chitosan-GMA-APB nanospheres have unique properties, such as good dispersibility, good biocompatibility and chemical stability, as well as augmented specificity with glycopeptides. Enrichment conditions were optimized by using trypsin digested glycoprotein horseradish peroxidase. The high specificity of chitosan-GMA-APB nanospheres was demonstrated by effectively enriching glycopeptides from a digest mixture of horseradish peroxidase and nonglycoproteins (bovine serum albumin (BSA)). (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000307616500007
资助机构"985 project" of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51953
专题北京大学基础医学院_北京大学系统生物医学研究所
北京大学医学信息学中心
北京大学医学部管理机构_医学部
北京大学基础医学院
北京大学医药卫生分析中心_研发办公室
作者单位1.Peking Univ, Med & Hlth Analyt Ctr, Prote Lab, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Syst Biomed, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zou, Xiajuan,Liu, Dan,Zhong, Lijun,et al. Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides[J]. CARBOHYDRATE POLYMERS,2012,90(2):799-804.
APA Zou, Xiajuan,Liu, Dan,Zhong, Lijun,Yang, Bin,Lou, Yaxin,&Yin, Yuxin.(2012).Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides.CARBOHYDRATE POLYMERS,90(2),799-804.
MLA Zou, Xiajuan,et al."Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides".CARBOHYDRATE POLYMERS 90.2(2012):799-804.
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