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学科主题: 基础医学
题名:
Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides
作者: Zou, Xiajuan1; Liu, Dan1; Zhong, Lijun1; Yang, Bin1; Lou, Yaxin1; Yin, Yuxin1,2
关键词: Chitosan-GMA-APB ; Glycopeptides ; Selective enrichment ; Nanosphere ; MALDI-TOF-MS
刊名: CARBOHYDRATE POLYMERS
发表日期: 2012-10-01
DOI: 10.1016/j.carbpol.2012.05.090
卷: 90, 期:2, 页:799-804
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]: Chemistry ; Polymer Science
关键词[WOS]: MASS-SPECTROMETRY ; LINKED OLIGOSACCHARIDES ; GLYCOPROTEOMICS ; CHROMATOGRAPHY ; PROTEINS
英文摘要:

In this study we describe a method for highly specific enrichment of glycopeptides with boronic acid-functionalized chitosan polymeric nanospheres and matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS). This is the first time chitosan has been used to create nanosphere support material for selective enrichment of glycopeptides by modification with glycidyl methacrylate (GMA) and derivatization with 3-aminophenylboronic acid (APB). Due to their multifunctional chemical moieties, these 20-100 nm chitosan-GMA-APB nanospheres have unique properties, such as good dispersibility, good biocompatibility and chemical stability, as well as augmented specificity with glycopeptides. Enrichment conditions were optimized by using trypsin digested glycoprotein horseradish peroxidase. The high specificity of chitosan-GMA-APB nanospheres was demonstrated by effectively enriching glycopeptides from a digest mixture of horseradish peroxidase and nonglycoproteins (bovine serum albumin (BSA)). (C) 2012 Elsevier Ltd. All rights reserved.

语种: 英语
项目资助者: "985 project" of China
WOS记录号: WOS:000307616500007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51953
Appears in Collections:基础医学院_北京大学系统生物医学研究所_期刊论文

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作者单位: 1.Peking Univ, Med & Hlth Analyt Ctr, Prote Lab, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Inst Syst Biomed, Beijing 100191, Peoples R China

Recommended Citation:
Zou, Xiajuan,Liu, Dan,Zhong, Lijun,et al. Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides[J]. CARBOHYDRATE POLYMERS,2012,90(2):799-804.
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