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Increase in extracellular cross-linking by tissue transglutaminase and reduction in expression of MMP-9 contribute differentially to focal segmental glomerulosclerosis in rats
Liu, SY; Li, YH; Zhao, HY; Chen, D; Huang, Q; Wang, SX; Zou, WZ; Zhang, YK; Li, XM; Huang, HC
关键词Focal And Segmental Glomerulosclerosis Tissue Transglutaminase Mmp-9 Fibronectin
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2006-03-01
DOI10.1007/s11010-005-9005-6
284期:1-2页:9-17
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]PUROMYCIN AMINONUCLEOSIDE ; GLOMERULAR SCLEROSIS ; TRANSGENIC MICE ; GROWTH-FACTORS ; MATRIX ; NEPHROPATHY ; MODULATION ; PODOCYTES ; KIDNEY ; CELLS
英文摘要

Tissue transglutaminase (tTG) is a Ca2+-dependent enzyme which stabilizes the extracellular matrix (ECM) through post-translational modification, and may play an important role in the pathogenesis of focal and segmental glomerulosclerosis (FSGS). Here, we have investigated whether tTG contributes to the glomerular ECM expansion in the puromycin aminonucleoside (PAN)-injection-induced experimental rat model of FSGS. The localization and expression of tTG, MMP-9 gelatinase, and the ECM component fibronectin (FN) in kidneys was determined by immunohistochemistry and measured by semi-quantitative analysis. Protein levels of tTG and MMP-9 were also analyzed by Western blotting.In situtransglutaminase activity was assayed by measurement of incorporated substrate and the immunofluorescence staining for the cross-linking product, epsilon-(gamma-glutamyl) lysine. Prominent proteinuria, a typical pathological feature of FSGS, was observed in PAN injection group rats. tTG immunoreactivity was located markedly in glomeruli and the levels of this protein in whole-kidney homogenates of PAN injection group rats were significantly increased (361 +/- 106% control, P < 0.05). Similarly, transglutaminase activity and epsilon-(gamma-glutamyl) lysine were also predominately located within glomeruli and were much more intense in the PAN-injected group than that in control animals. MMP-9 was also located primarily within glomeruli. In PAN-injected kidneys, protein levels of active MMP-9 were significantly reduced (59 +/- 27% control, P < 0.01), while pro-MMP-9 levels increased (148 +/- 42% control, P < 0.05). Remarkable expression of glomerular fibronectin (FN) was found in PAN injection group rats. Semi-quantitative analysis demonstrated this increased intensity of FN staining in the PAN-injected rats was 149 +/- 23% of the control values (P < 0.05). Enhanced cross-linking of ECM by tissue transglutaminase and decreased degradation due to reduced active MMP-9 expression may be at least partially responsible for the deposition of FN within injured glomeruli in experimental FSGS.

语种英语
WOS记录号WOS:000237510900002
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51963
专题北京大学第一临床医学院_肾脏内科
北京大学基础医学院
北京大学第一临床医学院_电镜室
北京大学第二临床医学院_消化科
北京大学第二临床医学院_肝胆外科
作者单位Peking Univ, Hosp 1, Div Nephrol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Liu, SY,Li, YH,Zhao, HY,et al. Increase in extracellular cross-linking by tissue transglutaminase and reduction in expression of MMP-9 contribute differentially to focal segmental glomerulosclerosis in rats[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2006,284(1-2):9-17.
APA Liu, SY.,Li, YH.,Zhao, HY.,Chen, D.,Huang, Q.,...&Huang, HC.(2006).Increase in extracellular cross-linking by tissue transglutaminase and reduction in expression of MMP-9 contribute differentially to focal segmental glomerulosclerosis in rats.MOLECULAR AND CELLULAR BIOCHEMISTRY,284(1-2),9-17.
MLA Liu, SY,et al."Increase in extracellular cross-linking by tissue transglutaminase and reduction in expression of MMP-9 contribute differentially to focal segmental glomerulosclerosis in rats".MOLECULAR AND CELLULAR BIOCHEMISTRY 284.1-2(2006):9-17.
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