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学科主题: 临床医学
题名:
Gene expression profile by blocking the SYT-SSX fusion gene in synovial sarcoma cells. Identification of XRCC4 as a putative SYT-SSX target gene
作者: Xie, YT1; Tornkvist, M1; Aalto, Y1; Nilsson, G1; Girnita, L1; Nagy, B1; Knuutila, S1; Larsson, O1
关键词: microarray ; antisense ; SYT-SSX ; synovial sarcoma
刊名: ONCOGENE
发表日期: 2003-10-23
DOI: 10.1038/sj.onc.1207153
卷: 22, 期:48, 页:7628-7631
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]: TRANSLOCATION ; SYT-SSX1 ; PROTEINS ; REPAIR ; CANCER ; D1
英文摘要:

Increasing evidence suggests that the SYT-SSX fusion gene plays an important role in synovial sarcoma development and progression. However, very little is known about the downstream targets of SYT-SSX. In this study, we used antisense oligonucleotides to block the expression of the SYT-SSX fusion gene in synovial sarcoma cells. By comparing SYT-SSX inhibited cells with noninhibited cells, the gene expression pro. le was analysed using cDNA microarray and established by real-time PCR. Herewith, using a filter containing 1176 cancer-relevant genes, we found that the DNA repair gene XRCC4 and the DNA mismatch repair gene MSH2 were downregulated, whereas the gene encoding for the serine/threonine protein kinase PRK (also known as CNK), and the macrophage inhibitory cytokine MICI ( also known as PLAB) were upregulated after the inhibition of SYT-SSX. In comparison, expression of the XRCC4 gene was undergoing the strongest alteration. Consistently, the protein expression of XRCC4 was found to be decreased after SYT-SSX inhibition, whereas there were no detectable changes for the other gene products. Our study provides some clues to elucidate the signaling pathways of the SYT-SSX fusion gene, as well as it demonstrates a valuable model system for search for other SYT-SSX targets.

语种: 英语
WOS记录号: WOS:000186113000010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51973
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Haartman Inst, Dept Med Genet, FIN-00014 Helsinki, Finland
2.Karolinska Hosp, Dept Orthoped, SE-17176 Stockholm, Sweden
3.Univ Helsinki, Cent Hosp, FIN-00014 Helsinki, Finland
4.Karolinska Hosp, Dept Pathol & Oncol, SE-17176 Stockholm, Sweden
5.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Surg, Beijing, Peoples R China

Recommended Citation:
Xie, YT,Tornkvist, M,Aalto, Y,et al. Gene expression profile by blocking the SYT-SSX fusion gene in synovial sarcoma cells. Identification of XRCC4 as a putative SYT-SSX target gene[J]. ONCOGENE,2003,22(48):7628-7631.
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