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学科主题临床医学
Gene expression profile by blocking the SYT-SSX fusion gene in synovial sarcoma cells. Identification of XRCC4 as a putative SYT-SSX target gene
Xie, YT1; Tornkvist, M1; Aalto, Y1; Nilsson, G1; Girnita, L1; Nagy, B1; Knuutila, S1; Larsson, O1
关键词Microarray Antisense Syt-ssx Synovial Sarcoma
刊名ONCOGENE
2003-10-23
DOI10.1038/sj.onc.1207153
22期:48页:7628-7631
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]TRANSLOCATION ; SYT-SSX1 ; PROTEINS ; REPAIR ; CANCER ; D1
英文摘要

Increasing evidence suggests that the SYT-SSX fusion gene plays an important role in synovial sarcoma development and progression. However, very little is known about the downstream targets of SYT-SSX. In this study, we used antisense oligonucleotides to block the expression of the SYT-SSX fusion gene in synovial sarcoma cells. By comparing SYT-SSX inhibited cells with noninhibited cells, the gene expression pro. le was analysed using cDNA microarray and established by real-time PCR. Herewith, using a filter containing 1176 cancer-relevant genes, we found that the DNA repair gene XRCC4 and the DNA mismatch repair gene MSH2 were downregulated, whereas the gene encoding for the serine/threonine protein kinase PRK (also known as CNK), and the macrophage inhibitory cytokine MICI ( also known as PLAB) were upregulated after the inhibition of SYT-SSX. In comparison, expression of the XRCC4 gene was undergoing the strongest alteration. Consistently, the protein expression of XRCC4 was found to be decreased after SYT-SSX inhibition, whereas there were no detectable changes for the other gene products. Our study provides some clues to elucidate the signaling pathways of the SYT-SSX fusion gene, as well as it demonstrates a valuable model system for search for other SYT-SSX targets.

语种英语
WOS记录号WOS:000186113000010
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51973
专题北京大学临床肿瘤学院
作者单位1.Haartman Inst, Dept Med Genet, FIN-00014 Helsinki, Finland
2.Karolinska Hosp, Dept Orthoped, SE-17176 Stockholm, Sweden
3.Univ Helsinki, Cent Hosp, FIN-00014 Helsinki, Finland
4.Karolinska Hosp, Dept Pathol & Oncol, SE-17176 Stockholm, Sweden
5.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Surg, Beijing, Peoples R China
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Xie, YT,Tornkvist, M,Aalto, Y,et al. Gene expression profile by blocking the SYT-SSX fusion gene in synovial sarcoma cells. Identification of XRCC4 as a putative SYT-SSX target gene[J]. ONCOGENE,2003,22(48):7628-7631.
APA Xie, YT.,Tornkvist, M.,Aalto, Y.,Nilsson, G.,Girnita, L.,...&Larsson, O.(2003).Gene expression profile by blocking the SYT-SSX fusion gene in synovial sarcoma cells. Identification of XRCC4 as a putative SYT-SSX target gene.ONCOGENE,22(48),7628-7631.
MLA Xie, YT,et al."Gene expression profile by blocking the SYT-SSX fusion gene in synovial sarcoma cells. Identification of XRCC4 as a putative SYT-SSX target gene".ONCOGENE 22.48(2003):7628-7631.
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