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A novel microbead-based microfluidic device for rapid bacterial identification and antibiotic susceptibility testing
He, J.1; Mu, X.2; Guo, Z.1; Hao, H.1; Zhang, C.3; Zhao, Z.4; Wang, Q.1
刊名EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
2014-12-01
DOI10.1007/s10096-014-2182-z
33期:12页:2223-2230
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases ; Microbiology
研究领域[WOS]Infectious Diseases ; Microbiology
关键词[WOS]POLY(DIMETHYLSILOXANE) ; DROPLETS ; SYSTEMS ; SEPSIS ; FLOW
英文摘要

Effective treatment of infectious diseases depends on the ability to rapidly identify the infecting bacteria and the use of sensitive antibiotics. The currently used identification assays usually take more than 72 h to perform and have a low sensitivity. Herein, we present a microbead-based microfluidic platform that is highly sensitive and rapid for bacterial detection and antibiotic sensitivity testing. The platform includes four units, one of which is used for bacterial identification and the other three are used for susceptibility testing. Our results showed that Escherichia coli O157 at a cell density range of 10(1)-10(5) CFU/mu L could be detected within 30 min. Additionally, the effects of three antibiotics on E. coli O157 were evaluated within 4-8 h. Overall, this integrated microbead-based microdevice provides a sensitive, rapid, reliable, and highly effective platform for the identification of bacteria, as well as antibiotic sensitivity testing.

语种英语
WOS记录号WOS:000345138300016
项目编号201202011
资助机构National Health and Family Planning Commission of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51989
专题北京大学第二临床医学院
作者单位1.Dalian Med Univ, Hosp 2, Dept Resp Med, Dalian 116023, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
3.Dalian Med Univ, Hosp 2, Dept Endocrinol Med, Dalian 116023, Peoples R China
4.Dalian Med Univ, Hosp 2, Dept Breast Surg Med, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
He, J.,Mu, X.,Guo, Z.,et al. A novel microbead-based microfluidic device for rapid bacterial identification and antibiotic susceptibility testing[J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES,2014,33(12):2223-2230.
APA He, J..,Mu, X..,Guo, Z..,Hao, H..,Zhang, C..,...&Wang, Q..(2014).A novel microbead-based microfluidic device for rapid bacterial identification and antibiotic susceptibility testing.EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES,33(12),2223-2230.
MLA He, J.,et al."A novel microbead-based microfluidic device for rapid bacterial identification and antibiotic susceptibility testing".EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES 33.12(2014):2223-2230.
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