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学科主题基础医学
PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway
Ma, Xi1; Zhao, Hongshan1; Shan, Jingxuan1; Long, Feng1; Chen, Yaoyao1; Chen, Yingyu1; Zhang, Yingmei1; Han, Xiao1; Ma, Dalong1
刊名MOLECULAR BIOLOGY OF THE CELL
2007-06-01
DOI10.1091/mbc.E06-07-0608
18期:6页:1965-1978
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]CEREBRAL CAVERNOUS MALFORMATIONS ; STE20-LIKE KINASE ; MAMMALIAN-CELLS ; CANCER CELLS ; GENE ; APOPTOSIS ; MUTATIONS ; PROTEINS ; CLONING ; SYSTEM
英文摘要

PDCD10 (programmed cell death 10, TFAR15), a novel protein associated with cell apoptosis has been recently implicated in mutations associated with Cerebral Cavernous Malformations (CCM). Yeast two-hybrid screening revealed that PDCD10 interacts with MST4, a member of Ste20-related kinases. This interaction was confirmed by coimmunoprecipitation and colocalization assays in mammalian cells. Furthermore, the co-overexpression of PDCD10 and MST4 promoted cell proliferation and transformation via modulation of the extracellular signal-regulated kinase (ERK) pathway. Potent short interfering RNAs (siRNAs) against PDCD10 (siPDCD10) and MST4 (siMST4) were designed to specifically inhibit the expression of PDCD10 and MST4 mRNA, respectively. The induction of siPDCD10 or siMST4 resulted in decreased expression of endogenous PDCD10 or MST4, which was accompanied by reduced ERK activity and attenuated cell growth and anchorage-independent growth. On the other hand, siMST4 had similar effects in PDCD10-overexpressed cells. And more importantly, we confirmed that either overexpressing or endogenous PDCD10 can increase the MST4 kinase activity in vitro. Our results demonstrated that PDCD10 modulation of ERK signaling was mediated by MST4, and PDCD10 could be a regulatory adaptor necessary for MST4 function, suggesting a link between cerebral cavernous malformation pathogenesis and the ERK-MAPK cascade via PDCD10/MST4.

语种英语
WOS记录号WOS:000246977600001
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被引频次:83[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51993
专题北京大学基础医学院_免疫学系
作者单位1.Shanghai Genom Inc, Shanghai 201203, Peoples R China
2.Peking Univ, Sch Basic Med, Dept Immunol, Beijing 100083, Peoples R China
3.Peking Univ, Human Dis Genom Ctr, Beijing 100083, Peoples R China
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Ma, Xi,Zhao, Hongshan,Shan, Jingxuan,et al. PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway[J]. MOLECULAR BIOLOGY OF THE CELL,2007,18(6):1965-1978.
APA Ma, Xi.,Zhao, Hongshan.,Shan, Jingxuan.,Long, Feng.,Chen, Yaoyao.,...&Ma, Dalong.(2007).PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway.MOLECULAR BIOLOGY OF THE CELL,18(6),1965-1978.
MLA Ma, Xi,et al."PDCD10 interacts with Ste20-related kinase MST4 to promote cell growth and transformation via modulation of the ERK pathway".MOLECULAR BIOLOGY OF THE CELL 18.6(2007):1965-1978.
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