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学科主题: 临床医学
题名:
Febrile seizure, but not hyperthermia alone, induces the expression of heme oxygenase-1 in rat cortex
作者: Yang, ZX; Qin, J; Du, JB; Bu, DF; Chang, XZ; Han, Y
关键词: febrile seizure ; heme oxygenase ; cortex
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2006-02-05
卷: 119, 期:3, 页:191-196
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: COMPETITIVE PCR ; BRAIN ; CONVULSIONS ; EPILEPSY ; CHILDREN ; PROTEIN ; HEMORRHAGE ; INDUCTION ; ISCHEMIA ; INJURY
英文摘要:

Background Febrile seizure (FS) is the most common seizure disorders. Approximately one third of children with a febrile seizure have recurrent events. The mechanism of FS remains unclear. Heme oxygenase-1 (HO-1) is a member of the heat shock proteins family and can be induced in the brain by various stresses, including hyperthemia and seizure. This study aimed at investigating the changes of HO-1 in the cortex of rats after recurrent FS.

Methods FS in rats was induced ten times, once every 2 days. In a bath of warm water, developing rats were randomly divided into two groups: control group (n=16) and warm water-treated group (n=50). The latter group was subdivided into hyperthermia group (n=19) and FS group (n=23). The expression and content of HO-1 mRNA in cortex were observed using in situ hybridization and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The content of HO-1 protein in cortex was measured using Western blotting.

Results HO-1 mRNA expression of cortex neurons in FS group was markedly increased in comparison with those in hyperthermia and control groups (P=0.00), however, there was no statistic difference between hyperthermia group and control group (P=0.16). The relative amount of HO-1 mRNA in cortex in FS group was increased by 53.13% and 96% in comparison with those in hyperthermia group and control group respectively (P=0.00), but there was no obvious difference between the later two groups (P=0.051). Western blotting analysis showed that the HO-1 protein content in cortex in FS group was increased by 198% and 246% in comparison with those in hyperthermia group and control group respectively (P=0.00). There was no obvious difference in HO-1 protein content between the later two groups (P=0.09).

Conclusions Recurrent FS in rats can cause the increase of HO-1 mRNA and protein in cortex which may be involved in the mechanism of FS. The short-time recurrent hyperthermia can not induce the increase of HO-1 mRNA and protein.

语种: 英语
WOS记录号: WOS:000235458700003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52000
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Yang, ZX,Qin, J,Du, JB,et al. Febrile seizure, but not hyperthermia alone, induces the expression of heme oxygenase-1 in rat cortex[J]. CHINESE MEDICAL JOURNAL,2006,119(3):191-196.
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