IR@PKUHSC  > 基础医学院  > 心血管所
学科主题基础医学
Human apolipoprotein A-I induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through ATP-binding cassette transporter A1
Liu, Donghui1,2; Ji, Liang1,2; Tong, Xunliang4; Pan, Bing1,2; Han, Jing-Yan5; Huang, Yining4; Chen, Y. Eugene6; Pennathur, Subramaniam6; Zhang, Youyi1,2,3; Zheng, Lemin1,2
关键词Apolipoprotein A-i High-density Lipoprotein Human Umbilical Vein Endothelial Cells
刊名AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
2011-09-01
DOI10.1152/ajpcell.00055.2011
301期:3页:C739-C748
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
资助者Natural Science Foundation of Beijing ; "973" National ST Major Project ; Natural Science Foundation of Fujian ; Peking University Health Science Center ; State Education Ministry of China ; Ministry of Science and Technology of China ; The Doris Duke Foundation ; American College of Rheumatology ; University of Michigan-Peking University Health Science Center Joint Institute ; program for "the Returned Overseas Chinese Scholars" ; program for "New Century Excellent Talents in Universities" ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; "973" National ST Major Project ; Natural Science Foundation of Fujian ; Peking University Health Science Center ; State Education Ministry of China ; Ministry of Science and Technology of China ; The Doris Duke Foundation ; American College of Rheumatology ; University of Michigan-Peking University Health Science Center Joint Institute ; program for "the Returned Overseas Chinese Scholars" ; program for "New Century Excellent Talents in Universities" ; National Natural Science Foundation of China
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]HIGH-DENSITY-LIPOPROTEINS ; CYTOKINE-INDUCED EXPRESSION ; MAPK SIGNALING PATHWAYS ; NITRIC-OXIDE SYNTHASE ; SMOOTH-MUSCLE-CELLS ; SPHINGOSINE 1-PHOSPHATE ; PLASMA-LIPOPROTEINS ; HDL ; CHOLESTEROL ; RECEPTORS
英文摘要

Liu D, Ji L, Tong X, Pan B, Han JY, Huang Y, Chen YE, Pennathur S, Zhang Y, Zheng L. Human apolipoprotein A-I induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through ATP-binding cassette transporter A1. Am J Physiol Cell Physiol 301: C739-C748, 2011. First published July 6, 2011; doi: 10.1152/ajpcell.00055.2011.-High-density lipoprotein (HDL) can induce cyclooxygenase-2 (COX-2) expression and prostacyclin I-2 (PGI-2) release in endothelial cells to exert multiple antiatherogenic functions. This effect has been attributed mainly to the role of sphingosine-1-phosphate (S1P) integrated in HDL. However, whether apolipoprotein A-I (apoA-I), the major apolipoprotein of HDL, could induce COX-2 expression and PGI-2 release still remains unclear. In the present study, we selectively delipidated HDL and confirmed that apoA-I could facilitate COX-2 expression and PGI-2 production in human umbilical vein endothelial cells (HUVECs). ApoA-I, but not trypsinized apoA-I, induced COX-2 expression in a time-and dose-dependent manner consistent with a key role for apoA-I in this process. Additionally, cotreatment of apoA-I with S1P further enhanced COX-2 expression and PGI-2 production in HUVECs. These effects triggered by apoA-I were not inhibited by pertussis toxin, consistent with SIP receptor independent pathway for apoA-I effect. Moreover, we demonstrated that the activation of p38 mitogen-activated protein kinase (MAPK), extracellular receptor kinase (ERK) 1/2, and JAK2 pathways by apoA-I was involved in the expression of COX-2 and the release of PGI-2 in HUVECs, and these effects were inhibited by their specific inhibitors, respectively. Small interfering RNA experiments showed that ATP binding-cassette transporter A1 (ABCA1) was required for COX-2 expression and PGI-2 release induced by apoA-I. Thus our results indicate that apoA-I induces COX-2 expression and PGI-2 release through ABCA1 and the activation of intracellular p38 MAPK, ERK1/2, as well as JAK2 pathways, and apoA-I can reinforce these effects with S1P in HUVECs. These novel effects of apoA-I could in part mediate antiatherogenic effects of HDL.

语种英语
所属项目编号7102104 ; 2010CB912504 ; 2011CB503900 ; 2009J01112 ; 70103-007 ; 20090001120039 ; 20100001120034 ; 2008ZX09401-006 ; 30900595 ; 30821001
资助者Natural Science Foundation of Beijing ; "973" National ST Major Project ; Natural Science Foundation of Fujian ; Peking University Health Science Center ; State Education Ministry of China ; Ministry of Science and Technology of China ; The Doris Duke Foundation ; American College of Rheumatology ; University of Michigan-Peking University Health Science Center Joint Institute ; program for "the Returned Overseas Chinese Scholars" ; program for "New Century Excellent Talents in Universities" ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; "973" National ST Major Project ; Natural Science Foundation of Fujian ; Peking University Health Science Center ; State Education Ministry of China ; Ministry of Science and Technology of China ; The Doris Duke Foundation ; American College of Rheumatology ; University of Michigan-Peking University Health Science Center Joint Institute ; program for "the Returned Overseas Chinese Scholars" ; program for "New Century Excellent Talents in Universities" ; National Natural Science Foundation of China
WOS记录号WOS:000294339400023
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52013
专题基础医学院_心血管所
作者单位1.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
4.Peking Univ, Hosp 1, Dept Neurol, Beijing 100191, Peoples R China
5.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China
6.Univ Michigan, Dept Med, Ann Arbor, MI 48109 USA
推荐引用方式
GB/T 7714
Liu, Donghui,Ji, Liang,Tong, Xunliang,et al. Human apolipoprotein A-I induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through ATP-binding cassette transporter A1[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2011,301(3):C739-C748.
APA Liu, Donghui.,Ji, Liang.,Tong, Xunliang.,Pan, Bing.,Han, Jing-Yan.,...&Zheng, Lemin.(2011).Human apolipoprotein A-I induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through ATP-binding cassette transporter A1.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,301(3),C739-C748.
MLA Liu, Donghui,et al."Human apolipoprotein A-I induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through ATP-binding cassette transporter A1".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 301.3(2011):C739-C748.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
C739.full.pdf(3008KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Donghui]的文章
[Ji, Liang]的文章
[Tong, Xunliang]的文章
百度学术
百度学术中相似的文章
[Liu, Donghui]的文章
[Ji, Liang]的文章
[Tong, Xunliang]的文章
必应学术
必应学术中相似的文章
[Liu, Donghui]的文章
[Ji, Liang]的文章
[Tong, Xunliang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: C739.full.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。