|APELIN IN EPIRETINAL FIBROVASCULAR MEMBRANES OF PATIENTS WITH RETINOPATHY OF PREMATURITY AND THE CHANGES AFTER INTRAVITREAL BEVACIZUMAB|
|Zhang, Yan1,2; Jiang, Yan-rong1,2; Lu, Qiang1,2; Yin, Hong1,2; Tao, Yong1,2|
|关键词||Apelin Retinopathy Of Prematurity Neo-vascularization Epiretinal Fibrovascular Membranes Bevacizumab|
|刊名||RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES|
|WOS标题词||Science & Technology|
|关键词[WOS]||ENDOTHELIAL GROWTH-FACTOR ; PROLIFERATIVE DIABETIC-RETINOPATHY ; RETINAL VEIN OCCLUSION ; FACTOR VEGF ; ANGIOGENIC FACTOR ; MACULAR EDEMA ; EXPRESSION ; RECEPTOR ; SYSTEM ; LIGAND|
Purpose: Apelin, a novel cytokine, was reported to regulate angiogenesis. The aim of this study was to investigate the correlation between apelin and retinopathy of prematurity (ROP), between apelin and the other known angiogenic cytokines including vascular endothelial growth factor (VEGF) and hypoxia-induced factor-1a (HIF-1a).
Methods: The study included 36 ROP patients who underwent vitrectomy. Previous intravitreal bevacizumab (IVB) was performed in 18 patients (IVB group). The other ROP eyes belonged to non-IVB group. Surgical removed membranes from 10 patients who underwent vitrectomy for idiopathic preretinal membranes or macular hole served as control. The expression of apelin and angiotensin-1-like receptor (APJ) in the excised membranes was examined by fluorescence immunostaining. Quantitative reverse transcription polymerase chain reaction was used to examine the expression of apelin, VEGF, and HIF-1a mRNA.
Results: The density of neovascularization in fibrovascular membranes was significantly correlated with the age and postconception age of ROP patients (r = -0.94, P < 0.01; r = -0.83, P = 0.04). In the non-IVB group, colocalization of the endothelial marker CD31 with the marker for apelin and colocalization of CD31 and APJ were observed. In the IVB group, staining of apelin and APJ were positive, whereas the staining of CD31 was negative. Expression of apelin mRNA, HIF-1a mRNA, and VEGF mRNA were significantly higher in ROP membranes than idiopathic epiretinal membranes. Expression of apelin mRNA and VEGF mRNA significantly correlate with HIF-1a mRNA (r = 0.64, P = 0.04; and r = 0.96, P < 0.01, respectively), but the expression of apelin mRNA did not significantly correlate with VEGF mRNA (r = 0.491, P = 0.15).
Conclusion: The apelin/APJ system may be involved in the development of retinal neovascularization of ROP. The present results showed that the effect of apelin was related to HIF-1a but independent with VEGF. RETINA 33:613-620, 2013
|项目编号||30901639 ; 81041005 ; 90561 ; 20110001110042 ; 20100001110073 ; 2009B04 ; 7112142|
|资助机构||National Natural Science Foundation of China ; European Foundation for the Study of Diabetes (EFSD) and Chinese Diabetes Society (CDS)/Lilly grant ; research fund for the doctoral program of higher education of China ; Beijing Novel Program ; Beijing Natural Science Foundation|
|作者单位||1.Minist Educ, Key Lab Vis Loss & Restorat, Beijing, Peoples R China|
2.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100044, Peoples R China
|Zhang, Yan,Jiang, Yan-rong,Lu, Qiang,et al. APELIN IN EPIRETINAL FIBROVASCULAR MEMBRANES OF PATIENTS WITH RETINOPATHY OF PREMATURITY AND THE CHANGES AFTER INTRAVITREAL BEVACIZUMAB[J]. RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES,2013,33(3):613-620.|
|APA||Zhang, Yan,Jiang, Yan-rong,Lu, Qiang,Yin, Hong,&Tao, Yong.(2013).APELIN IN EPIRETINAL FIBROVASCULAR MEMBRANES OF PATIENTS WITH RETINOPATHY OF PREMATURITY AND THE CHANGES AFTER INTRAVITREAL BEVACIZUMAB.RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES,33(3),613-620.|
|MLA||Zhang, Yan,et al."APELIN IN EPIRETINAL FIBROVASCULAR MEMBRANES OF PATIENTS WITH RETINOPATHY OF PREMATURITY AND THE CHANGES AFTER INTRAVITREAL BEVACIZUMAB".RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES 33.3(2013):613-620.|