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学科主题: 临床医学
题名:
Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells
作者: Huang, Lvzhen1; Yu, Wenzhen1; Li, Xiaoxin1; Xu, Yongsheng1; Niu, Lanjun1; He, Xiangjun2; Dong, Jianqiang2; Zheng Yan1
刊名: MOLECULAR VISION
发表日期: 2009-05-29
卷: 15, 期:112-13, 页:1057-1069
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]: RETINAL ENDOTHELIAL-CELLS ; MAGIC ROUNDABOUT ; ANGIOGENESIS ; RECEPTOR ; ROBO1/ROBO4 ; TARGET
英文摘要:

Purpose: Robo4, a member of the roundabout (Robo) family, acts as a neuronal guidance receptor and plays some role in vasculogenesis and angiogenesis. This study investigated the effect of Robo4 on the formation of fibrovascular membranes (FVMs) from patients with proliferative diabetic retinopathy and its roles in choroid-retina endothelial (RF/6A) and human retinal pigment epithelial (RPE) cells.

Methods: RT-PCR and immunohistochemistry were used to determine the levels of mRNA and the presence and distribution of Robo4 in FVMs. Small interfering RNA (siRNA) technology was used to knock down Robo4 expression and to study its effects on RF/6A and RPE cells in vitro. Cell proliferation, migration, spreading, cycling, and apoptosis were assessed with MTT assay, Boyden chamber assay, immunocytochemistry, and flow cytometry. Tube formation by RF/6A on Matrigel was also analyzed.

Results: The level of Robo4 mRNA was high in FVMs. Robo4 was expressed in the vessels and fibrous-like tissue co-immunostained for CD31 and GFAP, respectively. Robo4 siRNA knockdown inhibited cell proliferation and migration. Tube formation by RF/6A cells was also disturbed. Under hypoxic conditions, more apoptotic cells were evident among the knockdown cells than among the control cells (p < 0.01).

Conclusions: Robo4 may play a role in the formation of FVMs. Silencing the expression of Robo4 in RF/6A and RPE cells inhibited their proliferation and reduced their tolerance of hypoxic conditions, suggesting physiologic functions of Robo4 in the cells of the retina.

语种: 英语
所属项目编号: 2005CB724307
项目资助者: National Basic Research Program of China
WOS记录号: WOS:000267136400001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52042
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China

Recommended Citation:
Huang, Lvzhen,Yu, Wenzhen,Li, Xiaoxin,et al. Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells[J]. MOLECULAR VISION,2009,15(112-13):1057-1069.
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