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Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells
Huang, Lvzhen1; Yu, Wenzhen1; Li, Xiaoxin1; Xu, Yongsheng1; Niu, Lanjun1; He, Xiangjun2; Dong, Jianqiang2; Zheng Yan1
刊名MOLECULAR VISION
2009-05-29
15期:112-13页:1057-1069
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]RETINAL ENDOTHELIAL-CELLS ; MAGIC ROUNDABOUT ; ANGIOGENESIS ; RECEPTOR ; ROBO1/ROBO4 ; TARGET
英文摘要

Purpose: Robo4, a member of the roundabout (Robo) family, acts as a neuronal guidance receptor and plays some role in vasculogenesis and angiogenesis. This study investigated the effect of Robo4 on the formation of fibrovascular membranes (FVMs) from patients with proliferative diabetic retinopathy and its roles in choroid-retina endothelial (RF/6A) and human retinal pigment epithelial (RPE) cells.

Methods: RT-PCR and immunohistochemistry were used to determine the levels of mRNA and the presence and distribution of Robo4 in FVMs. Small interfering RNA (siRNA) technology was used to knock down Robo4 expression and to study its effects on RF/6A and RPE cells in vitro. Cell proliferation, migration, spreading, cycling, and apoptosis were assessed with MTT assay, Boyden chamber assay, immunocytochemistry, and flow cytometry. Tube formation by RF/6A on Matrigel was also analyzed.

Results: The level of Robo4 mRNA was high in FVMs. Robo4 was expressed in the vessels and fibrous-like tissue co-immunostained for CD31 and GFAP, respectively. Robo4 siRNA knockdown inhibited cell proliferation and migration. Tube formation by RF/6A cells was also disturbed. Under hypoxic conditions, more apoptotic cells were evident among the knockdown cells than among the control cells (p < 0.01).

Conclusions: Robo4 may play a role in the formation of FVMs. Silencing the expression of Robo4 in RF/6A and RPE cells inhibited their proliferation and reduced their tolerance of hypoxic conditions, suggesting physiologic functions of Robo4 in the cells of the retina.

语种英语
WOS记录号WOS:000267136400001
项目编号2005CB724307
资助机构National Basic Research Program of China
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52042
专题北京大学第二临床医学院_眼科
北京大学第二临床医学院_中心实验室
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
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Huang, Lvzhen,Yu, Wenzhen,Li, Xiaoxin,et al. Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells[J]. MOLECULAR VISION,2009,15(112-13):1057-1069.
APA Huang, Lvzhen.,Yu, Wenzhen.,Li, Xiaoxin.,Xu, Yongsheng.,Niu, Lanjun.,...&Zheng Yan.(2009).Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells.MOLECULAR VISION,15(112-13),1057-1069.
MLA Huang, Lvzhen,et al."Expression of Robo4 in the fibrovascular membranes from patients with proliferative diabetic retinopathy and its role in RF/6A and RPE cells".MOLECULAR VISION 15.112-13(2009):1057-1069.
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