IR@PKUHSC  > 北京大学基础医学院  > 医学信息学系
学科主题医学信息学
Sulindac inhibits tumor cell invasion by suppressing NF-kappa B-mediated transcription of microRNAs
Li, X.1; Gao, L.1; Cui, Q.2; Gary, B. D.1; Dyess, D. L.3; Taylor, W.1; Shevde, L. A.1; Samant, R. S.1; Dean-Colomb, W.1; Piazza, G. A.1; Xi, Y.1
关键词Sulindac Invasion Microrna Nf-kappa b
刊名ONCOGENE
2012-11-01
DOI10.1038/onc.2011.655
31期:48页:4979-4986
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]COLON-CANCER CELLS ; NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; FAMILIAL ADENOMATOUS POLYPOSIS ; HUMAN BREAST-CANCER ; COLORECTAL-CANCER ; REDUCED EXPRESSION ; E-CADHERIN ; RT-PCR ; METASTASIS ; APOPTOSIS
英文摘要

Non-steroidal anti-inflammatory drugs (NSAIDs) have been widely reported to display strong efficacy for cancer chemoprevention, although their mechanism of action is poorly understood. The most well-documented effects of NSAIDs include inhibition of tumor cell proliferation and induction of apoptosis, but their effect on tumor cell invasion has not been well studied. Here, we show that the NSAID, sulindac sulfide (SS) can potently inhibit the invasion of human MDA-MB-231 breast and HCT116 colon tumor cells in vitro at concentrations less than those required to inhibit tumor cell growth. To study the molecular basis for this activity, we investigated the involvement of microRNA (miRNA). A total of 132 miRNAs were found to be altered in response to SS treatment, including miR-10b, miR-17, miR-21 and miR-9, which have been previously implicated in tumor invasion and metastasis. We confirmed that these miRNA can stimulate tumor cell invasion and show that SS can attenuate their invasive effects by downregulating their expression. Employing luciferase and chromatin immunoprecipitation assays, NF-kappa B was found to bind the promoters of all four miRNAs to suppress their expression at the transcriptional level. We show that SS can inhibit the translocation of NF-kappa B to the nucleus by decreasing the phosphorylation of IKK beta and I kappa B. Analysis of the promoter sequences of the miRNAs suppressed by SS revealed that 81 of 115 sequences contained NF-kappa B-binding sites. These results show that SS can inhibit tumor cell invasion by suppressing NF-kappa B-mediated transcription of miRNAs. Oncogene (2012) 31, 4979-4986; doi:10.1038/onc.2011.655; published online 30 January 2012

语种英语
WOS记录号WOS:000311888600002
项目编号R01CA148817
资助机构National Cancer Institute
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52057
专题北京大学基础医学院_医学信息学系
作者单位1.Univ S Alabama, Dept Surg, Mobile, AL 36604 USA
2.Univ S Alabama, Mitchell Canc Inst, Mobile, AL 36604 USA
3.Peking Univ, Hlth Sci Ctr, Dept Biomed Informat, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Li, X.,Gao, L.,Cui, Q.,et al. Sulindac inhibits tumor cell invasion by suppressing NF-kappa B-mediated transcription of microRNAs[J]. ONCOGENE,2012,31(48):4979-4986.
APA Li, X..,Gao, L..,Cui, Q..,Gary, B. D..,Dyess, D. L..,...&Xi, Y..(2012).Sulindac inhibits tumor cell invasion by suppressing NF-kappa B-mediated transcription of microRNAs.ONCOGENE,31(48),4979-4986.
MLA Li, X.,et al."Sulindac inhibits tumor cell invasion by suppressing NF-kappa B-mediated transcription of microRNAs".ONCOGENE 31.48(2012):4979-4986.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
onc2011655a.pdf(1294KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, X.]的文章
[Gao, L.]的文章
[Cui, Q.]的文章
百度学术
百度学术中相似的文章
[Li, X.]的文章
[Gao, L.]的文章
[Cui, Q.]的文章
必应学术
必应学术中相似的文章
[Li, X.]的文章
[Gao, L.]的文章
[Cui, Q.]的文章
相关权益政策
暂无数据
收藏/分享
文件名: onc2011655a.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。