|Protective role of metallothionein in stress-induced gastric ulcer in rats|
|Jiang, Ping1,2; Chang, Lin1; Pan, Chun-Shui1; Qi, Yong-Fen2,3; Tang, Chao-Shu1,3|
|关键词||Metallothionein Gastric Ulcer|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||LIPID-PEROXIDATION ; MICE ; SUPPRESSION ; ETHANOL ; TISSUES ; DAMAGE ; ASSAY ; ZINC|
AIM: To illustrate the pathophysiological role of metallothionein (MT) in gastric ulcer induced by stress.
METHODS: Wistar rats underwent water-immersion-restraint (WIR) stress, ZnSO4 (an MT inducer) treatment, WIR+ ZnSO4 or WIR+ MT, and the ulcer index (UI) was estimated in excised stomach and liver tissues. The mRNA level of gastric MT was determined by semi-quantitative RT-PCR. The MT content in gastric and hepatic tissues was determined by Cd/hemoglobin affinity assay. The lipid peroxidation products malondialdehyde (MDA) and conjugated dienes (CD) were estimated by use of thiobarbituric acid reactive species and ultraviolet spectrophotometry.
RESULTS: WIR stress induced severe gastric mucosal lesions in rats. Compared with control rats, stressed rats had increased lipid peroxide content in serum and stomach and liver tissues. MDA content was increased by 34%, 21% and 29% and CD level by 270%, 83% and 28%, respectively. MT content in the stomach and liver was increased by 0.74- and 1.8-fold, and the MT-mRNA level in the stomach was increased by 26%. Pretreatment with ZnSO4 prevented gastric lesion development (the UI was 87% lower than that without pretreatment), and the MDA and CD content in serum and tissues was lower. The MT content in the liver was double in rats that were not pretreated, and the MT mRNA level in the stomach was 35% higher. MT administration 1 h before the WIR stress prevented gastric lesion development (the UI decreased by 47% compared with that in rats not pretreated), and the MDA and CD content in serum and tissues was significantly lower.
CONCLUSION: In WIR-stressed rats, the MT level was increased in serum and in stomach and liver tissues. Pre-administration of exogenous MT or pre-induction of endogenous MT can protect the gastric mucosa against stress-induced ulcers and inhibits the formation of stress-induced lipid peroxide. MT could have a gastroprotective effect and might be a new interventive and therapeutic target in stress-induced gastric ulcers. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
|资助机构||State Major Basic Research Development Program of the People&prime ; s Republic of China|
|作者单位||1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China|
2.Peking Univ, Hosp 1, Inst Cardiovasc Dis Res, Beijing 100034, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
|Jiang, Ping,Chang, Lin,Pan, Chun-Shui,et al. Protective role of metallothionein in stress-induced gastric ulcer in rats[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(18):2739-2743.|
|APA||Jiang, Ping,Chang, Lin,Pan, Chun-Shui,Qi, Yong-Fen,&Tang, Chao-Shu.(2005).Protective role of metallothionein in stress-induced gastric ulcer in rats.WORLD JOURNAL OF GASTROENTEROLOGY,11(18),2739-2743.|
|MLA||Jiang, Ping,et al."Protective role of metallothionein in stress-induced gastric ulcer in rats".WORLD JOURNAL OF GASTROENTEROLOGY 11.18(2005):2739-2743.|