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学科主题临床医学
Protective role of metallothionein in stress-induced gastric ulcer in rats
Jiang, Ping1,2; Chang, Lin1; Pan, Chun-Shui1; Qi, Yong-Fen2,3; Tang, Chao-Shu1,3
关键词Metallothionein Gastric Ulcer
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2005-05-14
11期:18页:2739-2743
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]LIPID-PEROXIDATION ; MICE ; SUPPRESSION ; ETHANOL ; TISSUES ; DAMAGE ; ASSAY ; ZINC
英文摘要

AIM: To illustrate the pathophysiological role of metallothionein (MT) in gastric ulcer induced by stress.

METHODS: Wistar rats underwent water-immersion-restraint (WIR) stress, ZnSO4 (an MT inducer) treatment, WIR+ ZnSO4 or WIR+ MT, and the ulcer index (UI) was estimated in excised stomach and liver tissues. The mRNA level of gastric MT was determined by semi-quantitative RT-PCR. The MT content in gastric and hepatic tissues was determined by Cd/hemoglobin affinity assay. The lipid peroxidation products malondialdehyde (MDA) and conjugated dienes (CD) were estimated by use of thiobarbituric acid reactive species and ultraviolet spectrophotometry.

RESULTS: WIR stress induced severe gastric mucosal lesions in rats. Compared with control rats, stressed rats had increased lipid peroxide content in serum and stomach and liver tissues. MDA content was increased by 34%, 21% and 29% and CD level by 270%, 83% and 28%, respectively. MT content in the stomach and liver was increased by 0.74- and 1.8-fold, and the MT-mRNA level in the stomach was increased by 26%. Pretreatment with ZnSO4 prevented gastric lesion development (the UI was 87% lower than that without pretreatment), and the MDA and CD content in serum and tissues was lower. The MT content in the liver was double in rats that were not pretreated, and the MT mRNA level in the stomach was 35% higher. MT administration 1 h before the WIR stress prevented gastric lesion development (the UI decreased by 47% compared with that in rats not pretreated), and the MDA and CD content in serum and tissues was significantly lower.

CONCLUSION: In WIR-stressed rats, the MT level was increased in serum and in stomach and liver tissues. Pre-administration of exogenous MT or pre-induction of endogenous MT can protect the gastric mucosa against stress-induced ulcers and inhibits the formation of stress-induced lipid peroxide. MT could have a gastroprotective effect and might be a new interventive and therapeutic target in stress-induced gastric ulcers. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000208098300009
项目编号2003CCA041
资助机构State Major Basic Research Development Program of the People&prime ; s Republic of China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52066
专题北京大学第一临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Inst Cardiovasc Dis Res, Beijing 100034, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Ping,Chang, Lin,Pan, Chun-Shui,et al. Protective role of metallothionein in stress-induced gastric ulcer in rats[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(18):2739-2743.
APA Jiang, Ping,Chang, Lin,Pan, Chun-Shui,Qi, Yong-Fen,&Tang, Chao-Shu.(2005).Protective role of metallothionein in stress-induced gastric ulcer in rats.WORLD JOURNAL OF GASTROENTEROLOGY,11(18),2739-2743.
MLA Jiang, Ping,et al."Protective role of metallothionein in stress-induced gastric ulcer in rats".WORLD JOURNAL OF GASTROENTEROLOGY 11.18(2005):2739-2743.
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