|Autoantibody production from a thymoma and a follicular dendritic cell sarcoma associated with paraneoplastic pemphigus|
|Wang, J1; Bu, DF1; Li, T1; Zheng, R1; Zhang, BX1; Chen, XX1; Zhu, XJ1|
|关键词||Autoantibody Follicular Dendritic Cell Sarcoma Paraneoplastic Pemphigus Thymoma|
|刊名||BRITISH JOURNAL OF DERMATOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||INDIRECT IMMUNOFLUORESCENCE ; CASTLEMANS-DISEASE ; PERIPLAKIN ; ENVOPLAKIN ; ANTIBODIES ; NEOPLASIA ; ANTIGENS ; PROTEINS ; FAMILY ; TUMORS|
Background Paraneoplastic pemphigus (PNP) is an autoimmune mucocutaneous disease. We previously reported that B cells in a Castleman tumour associated with PNP produced autoantibodies. However, it is uncertain whether the production of autoantibodies from the associated tumour is a common mechanism in PNP.
Objectives To investigate autoantibody production in a thymoma and a follicular dendritic cell sarcoma that were excised from two patients with PNP.
Mehods Tumour cells were cultured, and their surface markers were identified. Indirect immunofluorescence, immunoblotting and enzyme-linked immunosorbent assay (ELISA) using culture media from the tumours were used to detect PNP autoantibodies.
Results B cells with markers (CD22+, surface membrane IgG+ and surface membrane IgM+) of mature B lymphocytes constituted a proportion of cultured tumour cells in both tumours. Western blot showed that the medium from both the thymoma and the follicular dendritic cell sarcoma cells recognized 190-kDa periplakin and 210-kDa envoplakin bands of human epithelial proteins as well as recombinant linker regions of periplakin, envoplakin, desmoplakin and bullous pemphigoid antigen 1. ELISA was positive for antidesmoglein 3 antibody.
Conclusion The presence and localization in tumours of B-lymphocyte clones against proteins of the plakin family and desmoglein 3 in skin may not be confined to PNP with Castleman disease, but is possibly a common mechanism in PNP associated with various tumours.
|作者单位||1.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China|
2.Peking Univ, Hosp 1, Dept Pathol, Beijing 100034, Peoples R China
|Wang, J,Bu, DF,Li, T,et al. Autoantibody production from a thymoma and a follicular dendritic cell sarcoma associated with paraneoplastic pemphigus[J]. BRITISH JOURNAL OF DERMATOLOGY,2005,153(3):558-564.|
|APA||Wang, J.,Bu, DF.,Li, T.,Zheng, R.,Zhang, BX.,...&Zhu, XJ.(2005).Autoantibody production from a thymoma and a follicular dendritic cell sarcoma associated with paraneoplastic pemphigus.BRITISH JOURNAL OF DERMATOLOGY,153(3),558-564.|
|MLA||Wang, J,et al."Autoantibody production from a thymoma and a follicular dendritic cell sarcoma associated with paraneoplastic pemphigus".BRITISH JOURNAL OF DERMATOLOGY 153.3(2005):558-564.|