北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 感染疾病科  > 期刊论文
学科主题: 临床医学
题名:
Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43
作者: Mourez, Thomas1; Vabret, Astrid1; Han, Yang1; Dina, Julia1; Legrand, Loic1; Corbet, Sandrine1; Freymuth, Francois1
关键词: coronavirus ; OC43 strain ; nucleocapsid protein ; baculovirus ; insect cells ; Western blot
刊名: JOURNAL OF VIROLOGICAL METHODS
发表日期: 2007-02-01
DOI: 10.1016/j.jviromet.2006.09.024
卷: 139, 期:2, 页:175-180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
关键词[WOS]: RECOMBINANT BACULOVIRUSES ; HUMAN CORONAVIRUS ; IDENTIFICATION ; INFECTION ; PNEUMONIA ; DIAGNOSIS ; ELISA ; GENE ; OC43
英文摘要:

The nucleocapsid (N) gene of human coronavirus strain OC43 (HCoV-OC43) was amplified by reverse transcriptase-polymerase chain reaction, and cloned in pENTR (TM)/D-TOPO (R) plasmid. This plasmid containing the N gene was recombined with in a BaculoDirect (TM) baculovirus DNA designed in order to express N protein in fusion with a C-terminal polyhistidine tag containing V5 epitope. Sf21 cells were transfected with recombinant baculovirus DNA. Recombinant N protein was extracted from infected cells, analysed by SDS-PAGE and Western blot, and purified by Ni2+ affinity procedure. Sera from 100 healthcare workers and five 2-3-year-old children were tested in a Western blot assay using the purified recombinant N protein. All of the sera from adults and two of the sera from children have a positive result. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000244090300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52079
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Hosp Caen, Lab Human & Mol Virol, F-14033 Caen, France
2.Peking Univ, Med Coll Hosp, Dept Infect Dis, Beijing 100871, Peoples R China

Recommended Citation:
Mourez, Thomas,Vabret, Astrid,Han, Yang,et al. Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43[J]. JOURNAL OF VIROLOGICAL METHODS,2007,139(2):175-180.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Mourez, Thomas]'s Articles
[Vabret, Astrid]'s Articles
[Han, Yang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Mourez, Thomas]‘s Articles
[Vabret, Astrid]‘s Articles
[Han, Yang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace