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学科主题: 临床医学
题名:
Neural precursors derived from human embryonic stem cells
作者: Peng, HM1; Chen, G1
关键词: human embryonic stem cell ; differentiation ; neural precursor
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2005-06-01
DOI: 10.1360/062004-83
卷: 48, 期:3, 页:295-299
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: IN-VITRO ; HUMAN BLASTOCYSTS ; SPINAL-CORD ; TRANSPLANTATION ; DIFFERENTIATION ; NEURONS ; LINES
英文摘要:

Human embryonic stem (hES) cells provide a promising supply of specific cell types for transplantation therapy. We presented here the method to induce differentiation of purified neural precursors from hES cells. hES cells (Line PKU-1 and Line PKU-2) were cultured in suspension in bacteriological Petri dishes, which differentiated into cystic embryoid bodies (EBs). The EBs were then cultured in N-2 medium containing bFGF in poly-L-lysine-coated tissue culture dishes for two weeks. The central, small cells with 2-3 short processes of the spreading outgrowth were isolated mechanically and replated. The resulting neurospheres were cultured in suspension for 10 days, then dissociated into single cell suspension with a Pasteur pipette and plated. Cells grew vigorously in an attached way and were passed every 4-5 days. Almost all the cells were proved nestin positive by immunostaining. Following withdrawal of bFGF, they differentiated into neurons expressing beta-tubulin isotype(III), GABA, serotonin and synaptophysin. Through induction of PDGF-AA, they differentiated into astrocytes expressing GFAP and oligodendrocytes expressing O-4. The results showed that hES cells can differentiate into typical neural precursors expressing the specific marker nestin and capable of generating all three cell types of the central nervous system (CNS) in vitro.

语种: 英语
WOS记录号: WOS:000230554700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52087
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100083, Peoples R China
2.Gen Hosp PLA, Dept Obstet & Gynecol, Beijing 100853, Peoples R China

Recommended Citation:
Peng, HM,Chen, G. Neural precursors derived from human embryonic stem cells[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2005,48(3):295-299.
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