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Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone
Zhao, Minzhi2; An, Mingrui3; Wang, Qingsong3; Liu, Xiaochen2; Lai, Wenjia3; Zhao, Xuyang3; Wei, Shicheng1,2; Ji, Jianguo2,3
关键词Biomaterial Biocompatibility Silac Polyetheretherketone (Peek) Titanium Proteomics
刊名JOURNAL OF PROTEOMICS
2012-06-27
DOI10.1016/j.jprot.2012.03.033
75期:12页:3560-3573
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MICROARRAY DATA ; PROTEIN ; BIOMATERIAL ; GENE
英文摘要

Commercially pure titanium (cpTi) and polyetheretherketone (PEEK) are widely used surface-modified implant materials in orthopedics and dental therapeutics. However, there still has not been comprehensive biocompatibility evaluation of them at molecular level. By employing stable isotope labeling with amino acids in cell culture (SILAC), we profiled the dynamic protein expression changes in human osteoblast-like MG-63 cells cultured on cpTi and PEEK, respectively. About 2000 proteins were quantified and 400 proteins showed substantial alterations in expression levels upon each material treatment Notably, the extent of alterations diminished as the contact prolonged, which suggested adaptive response to the bioinert materials. Similar patterns of expression changes were observed for both cpTi and PEEK. The representative pathways reflected the regulation of biosynthesis, metabolism and cell adhesion in the adaptive process. In addition, PEEK showed stronger inhibition on mRNA processing, which explained the lower proliferation rate of the cells cultured on PEEK. Our results indicated that the widely used bioinert materials cpTi and PEEK could individually induce a cooperative response involving a wide panel of proteins and pathways. This study has established a basis for better understanding the biocompatibility of surface-modified implant biomaterials at molecular level. (c) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000307086500014
项目编号2007CB936103 ; 2010CB912203 ; 2011CB915504 ; 90919023 ; 30970652
资助机构National Key Basic Research Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52091
专题北京大学口腔医学院
北京大学基础医学院
作者单位1.Peking Univ, Sch Stomatol, Ctr Biomat & Med Devices, Beijing 100081, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China
3.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
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Zhao, Minzhi,An, Mingrui,Wang, Qingsong,et al. Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone[J]. JOURNAL OF PROTEOMICS,2012,75(12):3560-3573.
APA Zhao, Minzhi.,An, Mingrui.,Wang, Qingsong.,Liu, Xiaochen.,Lai, Wenjia.,...&Ji, Jianguo.(2012).Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone.JOURNAL OF PROTEOMICS,75(12),3560-3573.
MLA Zhao, Minzhi,et al."Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone".JOURNAL OF PROTEOMICS 75.12(2012):3560-3573.
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