|Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study|
|Wang, Xueqian1; Tian, Xiuyun1; Liu, Fangfang1; Zhao, Yiqiang1; Sun, Min1; Chen, Dafang3; Lu, Changdong4; Wang, Zhong4; Shi, Xiaotian4; Zhang, Qingying5; Zhang, Donghong5; Shen, Zhongying5; Li, Feng6; Harris, Curtis C.7; Cai, Hong1; Ke, Yang1,2|
|WOS标题词||Science & Technology|
|关键词[WOS]||HUMAN-PAPILLOMAVIRUS DNA ; POLYMERASE-CHAIN-REACTION ; SQUAMOUS-CELL CARCINOMAS ; HIGH-RISK AREA ; CHINA ; LINXIAN ; ASSOCIATION ; INFECTION ; TYPE-16 ; ABSENCE|
Background: HPV has been found repeatedly in esophageal carcinoma tissues. However, reported detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types, and geographic regions of sample origin have been suggested as potential causes of this discrepancy.
Methods: HPV L1 DNA and HPV genotypes were evaluated in 435 esophageal carcinoma specimens collected from four geographic regions with different ethnicities including Anyang in north China, Shantou in south China, Xinjiang in west China, and the United States. The HPV L1 fragment was detected using SPF1/GP6+ primers. HPV genotyping was performed using genotype specific PCR.
Results: Two hundred and forty four of 435 samples (56.1%) tested positive for HPV L1. Significant differences in detection rate were observed neither among the three areas of China nor between China and the US. HPV6, 16, 18, 26, 45, 56, 57, and 58 were identified in L1 positive samples. HPV16 and 57 were the most common types in all regions, followed by HPV26 and HPV18.
Conclusions: HPV infection is common in esophageal carcinoma independent of region and ethnic group of origin. Findings in this study raise the possibility that HPV is involved in esophageal carcinogenesis. Further investigation with a larger sample size over broader geographic areas may be warranted.
|项目编号||30430710 ; 30872937 ; 2006AA2Z467 ; 2006AA02A403 ; Z09050703080902|
|资助机构||Natural Science Foundation of China ; "863" Key Projects of National Ministry of Science and Technology ; Beijing Project on Science and Technology Commission|
|作者单位||1.Shihezi Med Sch, Shihezi 832002, Xinjiang, Peoples R China|
2.Anyang Canc Hosp, Anyang 455000, Henan, Peoples R China
3.Peking Univ, Beijing Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Minist Educ,Sch Oncol, Beijing 100142, Peoples R China
4.Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100191, Peoples R China
5.Peking Univ, Sch Publ Hlth, Hlth Sci Ctr, Beijing 100191, Peoples R China
6.Shantou Univ, Sch Med, Shantou 515031, Shandong, Peoples R China
7.NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA
|Wang, Xueqian,Tian, Xiuyun,Liu, Fangfang,et al. Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study[J]. BMC CANCER,2010,10.|
|APA||Wang, Xueqian.,Tian, Xiuyun.,Liu, Fangfang.,Zhao, Yiqiang.,Sun, Min.,...&Ke, Yang.(2010).Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study.BMC CANCER,10.|
|MLA||Wang, Xueqian,et al."Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study".BMC CANCER 10(2010).|