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学科主题临床医学
Gonadotropin-Mediated Dynamic Alterations During Bovine Oocyte Maturation In Vitro
Lu, Cui-Ling1,2,3; Wang, Tian-Ren1,2,3; Yan, Li-Ying1,2,3; Xia, Xi1,2,3; Zhu, Xiao-Hui1,2,3; Li, Rong1,2,3; Zhao, Hong-Cui1,2,3; Yan, Jie1,2,3; Yin, Tai-Lang1,2,3; Jin, Hong-Yan1,2,3; Zhang, Yan1,2,3; Zhang, Wen-Xin1,2,3; Feng, Huai-Liang4; Qiao, Jie1,2,3
关键词Gonadotropin Methylation Mitochondria Oocyte
刊名BIOLOGY OF REPRODUCTION
2014-08-01
DOI10.1095/biolreprod.114.117945
91期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Reproductive Biology
研究领域[WOS]Reproductive Biology
关键词[WOS]FOLLICLE-STIMULATING-HORMONE ; IMPRINTED GENES ; DNA METHYLATION ; DEVELOPMENTAL COMPETENCE ; PREIMPLANTATION EMBRYO ; MOUSE OOCYTES ; FERTILIZATION ; TECHNOLOGIES ; CULTURE ; HUMANS
英文摘要

Gonadotropins have been widely used in human-assisted reproduction and animal science for the past four decades. However, the effects of gonadotropins on oocyte maturation at the molecular and biochemical levels are poorly understood. To determine the effects of gonadotropins (recombinant follicle stimulating hormone and urinary human menopausal gonadotropin) on oocyte maturation, we used the bovine oocyte in vitro maturation model. First, we studied the effects of increasing gonadotropin concentrations on nuclear maturation and mitochondrial function in oocytes. Gonadotropins at concentrations of 0.075 and 0.75 IU/ml improved nuclear maturation and increased inner mitochondrial membrane potential and ATP levels; however, there were no beneficial effects at concentrations of 7.5 and 75 IU/ml. Second, we studied the effects of increasing gonadotropin concentrations on the status of methylation in matured (MII) oocytes. Aberrant methylation and demethylation of H19, SNRPN, and PEG3 genes were observed in MII oocytes at all concentrations except 0.075 IU/ml. The expression of genes that function in spindle formation, cell cycle control, and methylation was also downregulated by high gonadotropin concentrations. In conclusion, we established the optimal gonadotropin concentration (i.e., 0.075 IU/ml) to be used for bovine oocyte in vitro maturation studies. These results may provide a guide for clinical stimulation protocols and help to reduce the risks associated with gonadotropin administration during in vitro fertilization treatment.

语种英语
WOS记录号WOS:000341300400020
项目编号31128013/C120205 ; 81000275 ; 2011CB944500 ; 39252
资助机构Joint Research Fund for Overseas Natural Science of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; Merck and Co. Inc.
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52111
专题北京大学第三临床医学院
北京大学第三临床医学院_妇产科
北京大学第三临床医学院_生殖医学中心
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
4.Cornell Univ, Affiliated Weill Med Coll, New York Hosp Queens, Dept Obstet & Gynecol, New York, NY 10021 USA
推荐引用方式
GB/T 7714
Lu, Cui-Ling,Wang, Tian-Ren,Yan, Li-Ying,et al. Gonadotropin-Mediated Dynamic Alterations During Bovine Oocyte Maturation In Vitro[J]. BIOLOGY OF REPRODUCTION,2014,91(2).
APA Lu, Cui-Ling.,Wang, Tian-Ren.,Yan, Li-Ying.,Xia, Xi.,Zhu, Xiao-Hui.,...&Qiao, Jie.(2014).Gonadotropin-Mediated Dynamic Alterations During Bovine Oocyte Maturation In Vitro.BIOLOGY OF REPRODUCTION,91(2).
MLA Lu, Cui-Ling,et al."Gonadotropin-Mediated Dynamic Alterations During Bovine Oocyte Maturation In Vitro".BIOLOGY OF REPRODUCTION 91.2(2014).
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