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学科主题: 临床医学
题名:
Ischemic preconditioning increases GSK-3 beta/beta-catenin levels and ameliorates liver ischemia/reperfusion injury in rats
作者: Yan, Yichao1; Li, Guangying2; Tian, Xiaofeng3; Ye, Yingjiang1; Gao, Zhidong1; Yao, Jihong4; Zhang, Feng3; Wang, Shan1
关键词: ischemia/reperfusion ; ischemia preconditioning ; glycogen synthase kinase-3 beta ; b-catenin
刊名: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
发表日期: 2015-06-01
DOI: 10.3892/ijmm.2015.2153
卷: 35, 期:6, 页:1625-1632
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: GLYCOGEN-SYNTHASE KINASE ; BETA-CATENIN ; REPERFUSION INJURY ; HEPATOCYTE PROLIFERATION ; DOWN-REGULATION ; MECHANISM ; APOPTOSIS ; PATHWAY ; MYOCARDIUM ; INHIBITION
英文摘要:

Ischemic preconditioning (IPC) ameliorates ischemia/reperfusion (I/R) injury in a number of organs, and the glycogen synthase kinase-3 beta (GSK-3 beta)/beta-catenin signaling pathway regulates I/R-induced proliferation and apoptosis in the central nervous system and heart. However, the function of this signaling pathway in IPC during liver I/R remains unclear. Thus, in this study, we aimed to investigte the role of the GSK-3 beta/beta-catenin pathway during I/R and following ischemic preconditioning. For this purpose, 30 Sprague-Dawley rats were randomly divided into the sham-operated, the I/R and the IPC groups (n=10). Following reperfusion, liver pathology, as well as alanine aminotransferase (ALT), aspartate aminotransferase (AST), maleic dialdehyde (MDA) and superoxide dismutase (SOD) levels were assessed. Western blot analysis was performed to quantify the GSK-3 beta, Ser9-phospho-GSK-3 beta (p-GSK-3 beta), cytosolic and nuclear beta-catenin, vascular endothelial growth factor (VEGF), Bcl-2 and survivin levels. In addition, the Bcl-2 and survivin mRNA levels were assessed by RT-qPCR. Compared with the sham-operated group, I/R increased serum ALT, AST and MDA activity and decreased SOD levels, while IPC significantly decreased serum ALT, AST and MDA activity and increased SOD levels, compared with the I/R group. Simultaneously, I/R increased p-GSK-3 beta protein expression, and decreased Bcl-2 and survivin protein and mRNA levels. IPC further increased the protein expression of p-GSK-3 beta, and also increased cytosolic and nuclear beta-catenin and VEGF expression compared with the I/R group; the expression of Bcl-2 and survivin was also increased by IPC, both at the mRNA and protein level. The total GSK-3 beta expression remained unaltered in all the groups. In conclusion, our data demonstrate that IPC exerts protective effects against liver injury induced by I/R and activates the GSK-3 beta/beta-catenin signaling pathway.

语种: 英语
WOS记录号: WOS:000354081100016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52113
Appears in Collections:北京大学第二临床医学院_肿瘤科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Lab Surg Oncol, Dept Gastroenterol Surg, Beijing 100044, Peoples R China
2.Peoples Liberat Army 202 Hosp, Dept Gynaecol & Obstet, Shenyang 110002, Liaoning, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Gen Surg, Dalian 116027, Liaoning, Peoples R China
4.Dalian Med Univ, Dept Pharmacol, Dalian 116044, Liaoning, Peoples R China

Recommended Citation:
Yan, Yichao,Li, Guangying,Tian, Xiaofeng,et al. Ischemic preconditioning increases GSK-3 beta/beta-catenin levels and ameliorates liver ischemia/reperfusion injury in rats[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2015,35(6):1625-1632.
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