学科主题临床医学
BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells
Pan, Xiao-Ben1; Qu, Xiao-Wang2; Jiang, Dong1; Zhao, Xing-Liang1; Han, Jin-Chao1; Wei, Lai1
关键词Hcv Bst2 Interferon
刊名ANTIVIRAL RESEARCH
2013-04-01
DOI10.1016/j.antiviral.2013.01.009
98期:1页:54-60
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Virology
研究领域[WOS]Pharmacology & Pharmacy ; Virology
关键词[WOS]INTERFERON-INDUCED PROTEIN ; PARTICLE RELEASE ; ALPHA-INTERFERON ; RNA REPLICATION ; TETHERIN ; IDENTIFICATION ; MECHANISMS ; REPLICON ; INFECTIONS ; EXPRESSION
英文摘要

Hepatitis C virus (HCV) infection is a common cause of chronic hepatitis and is currently treated with alpha interferon (IFN-alpha)-based therapies. IFN-induced cell membrane protein BST2 (also known as CD317, HM1.24 or tetherin) has been reported to tether a broad range of lipid-enveloped viruses on cell surfaces. However, whether HCV is sensitive to BST2 remains controversial. Here we established a Huh7.5-BST2-TO cell line, in which BST2 expression is regulated by tetracycline. Our results showed that the effect of BST2 on inhibiting HCV production was dependent on its expression level. Highly expressed BST2 reduced the yield of cell-free HCV virions but did not affect the efficiency of HCV infection and genome replication. Co-localization of HCV core protein and BST2 was detected by immunofluorescence in certain cells with high expression, but not in cells with low BST2 expression. Furthermore, inhibition of IFN-alpha induced BST2 expression in Huh7.5 cells by siRNA technology slightly reduced the antiviral response of the cytokine against HCV, but only at low IFN-alpha concentration. While overexpression of BST2 inhibited HCV replication in this system, BST2 is therefore not likely to be a major contributor to the antiviral effect of IFN-alpha. (c) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000318466200008
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被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52115
专题北京大学第二临床医学院_北京大学肝病研究所
北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis & Immunotherapy Liver D, Beijing 100044, Peoples R China
2.Drexel Univ, Dept Microbiol & Immunol, Drexel Inst Biotechnol & Virol Res, Philadelphia, PA USA
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Pan, Xiao-Ben,Qu, Xiao-Wang,Jiang, Dong,et al. BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells[J]. ANTIVIRAL RESEARCH,2013,98(1):54-60.
APA Pan, Xiao-Ben,Qu, Xiao-Wang,Jiang, Dong,Zhao, Xing-Liang,Han, Jin-Chao,&Wei, Lai.(2013).BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells.ANTIVIRAL RESEARCH,98(1),54-60.
MLA Pan, Xiao-Ben,et al."BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells".ANTIVIRAL RESEARCH 98.1(2013):54-60.
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