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学科主题: 临床医学
题名:
BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells
作者: Pan, Xiao-Ben1; Qu, Xiao-Wang2; Jiang, Dong1; Zhao, Xing-Liang1; Han, Jin-Chao1; Wei, Lai1
关键词: HCV ; BST2 ; Interferon
刊名: ANTIVIRAL RESEARCH
发表日期: 2013-04-01
DOI: 10.1016/j.antiviral.2013.01.009
卷: 98, 期:1, 页:54-60
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Virology
研究领域[WOS]: Pharmacology & Pharmacy ; Virology
关键词[WOS]: INTERFERON-INDUCED PROTEIN ; PARTICLE RELEASE ; ALPHA-INTERFERON ; RNA REPLICATION ; TETHERIN ; IDENTIFICATION ; MECHANISMS ; REPLICON ; INFECTIONS ; EXPRESSION
英文摘要:

Hepatitis C virus (HCV) infection is a common cause of chronic hepatitis and is currently treated with alpha interferon (IFN-alpha)-based therapies. IFN-induced cell membrane protein BST2 (also known as CD317, HM1.24 or tetherin) has been reported to tether a broad range of lipid-enveloped viruses on cell surfaces. However, whether HCV is sensitive to BST2 remains controversial. Here we established a Huh7.5-BST2-TO cell line, in which BST2 expression is regulated by tetracycline. Our results showed that the effect of BST2 on inhibiting HCV production was dependent on its expression level. Highly expressed BST2 reduced the yield of cell-free HCV virions but did not affect the efficiency of HCV infection and genome replication. Co-localization of HCV core protein and BST2 was detected by immunofluorescence in certain cells with high expression, but not in cells with low BST2 expression. Furthermore, inhibition of IFN-alpha induced BST2 expression in Huh7.5 cells by siRNA technology slightly reduced the antiviral response of the cytokine against HCV, but only at low IFN-alpha concentration. While overexpression of BST2 inhibited HCV replication in this system, BST2 is therefore not likely to be a major contributor to the antiviral effect of IFN-alpha. (c) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30972605 ; 30700697 ; BMU20110272
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University (NCET)
WOS记录号: WOS:000318466200008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52115
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis & Immunotherapy Liver D, Beijing 100044, Peoples R China
2.Drexel Univ, Dept Microbiol & Immunol, Drexel Inst Biotechnol & Virol Res, Philadelphia, PA USA

Recommended Citation:
Pan, Xiao-Ben,Qu, Xiao-Wang,Jiang, Dong,et al. BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells[J]. ANTIVIRAL RESEARCH,2013,98(1):54-60.
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