IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
Ephrin-A4 Is Involved in Retinal Neovascularization by Regulating the VEGF Signaling Pathway
Du, Wei; Yu, Wenzhen; Huang, Lvzhen; Zhao, Min; Li, Xiaoxin
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2012-04-01
DOI10.1167/iovs.11-8788
53期:4页:1990-1998
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者National Basic Research Program of China (973 Program) ; Peking University People ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Peking University People ; National Natural Science Foundation of China
研究领域[WOS]Ophthalmology
关键词[WOS]ENDOTHELIAL-GROWTH-FACTOR ; EPHA RECEPTOR ; OCULAR NEOVASCULARIZATION ; INDUCED ANGIOGENESIS ; A4 EXPRESSION ; ANIMAL-MODELS ; RETINOPATHY ; MICE ; OSTEOSARCOMA ; INTERFERENCE
英文摘要

PURPOSE. Retinal neovascularization (NV) is a major cause of blindness. Recent research suggests that factors other than VEGF participate in this process. This study aimed to determine the role of ephrin-A4 in retinal NV.

METHODS. The expression and effect of ephrin-A4 was investigated in a mouse model of oxygen-induced retinopathy (OIR) and the RF/6A retina endothelial cell line. Ephrin-A4 expression and VEGF signaling pathway phosphorylation were determined by PCR, immunohistochemistry, and western blot analyses. ShRNA was used to silence ephrin-A4 in vitro and in vivo. Retinal flat mounts and tube formation assays were performed to evaluate ephrin-A4 function in the NV process in vivo and in vitro.

RESULTS. Ephrin-A4 was overexpressed in the retina of OIR mice and in RF/6A and RPE cells after CoCl2 stimulation. In vitro, Ephrin-A4/Fc treatment significantly increased the tube number of RF/6A cells on a membrane preparation and the phosphorylation levels of VEGR2, Akt1, and ERK1/2 in RF/6A cells. Moreover, ephrin-A4 knockout markedly suppressed pathologic neovascularization in vivo and inhibited the proliferation and tube formation capacity of RF/6A cells in vitro. Furthermore, in the absence of ephrin-A4, the phosphorylation of VEGFR2, Akt1, and ERK1/2 was defective under VEGF(165) stimulation, and the proangiogenic function of VEGF165 was also compromised.

CONCLUSIONS. This study suggests that ephrin-A4 plays an important role in retinal NV and is a potential target against retinal NV. The proangiogenic function of ephrin-A4 may be linked to its crucial role in the VEGF signaling pathway. (Invest Ophthalmol Vis Sci. 2012;53:1990-1998) DOI:10.1167/iovs.11-8788

语种英语
所属项目编号2011CB510200 ; 65288 ; RDB2010-26 65289 ; 81170854
资助者National Basic Research Program of China (973 Program) ; Peking University People ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Peking University People ; National Natural Science Foundation of China
WOS记录号WOS:000303669400036
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52122
专题北京大学第二临床医学院_眼科
作者单位Peking Univ, Peoples Hosp, Dept Ophthalmol, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Du, Wei,Yu, Wenzhen,Huang, Lvzhen,et al. Ephrin-A4 Is Involved in Retinal Neovascularization by Regulating the VEGF Signaling Pathway[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2012,53(4):1990-1998.
APA Du, Wei,Yu, Wenzhen,Huang, Lvzhen,Zhao, Min,&Li, Xiaoxin.(2012).Ephrin-A4 Is Involved in Retinal Neovascularization by Regulating the VEGF Signaling Pathway.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,53(4),1990-1998.
MLA Du, Wei,et al."Ephrin-A4 Is Involved in Retinal Neovascularization by Regulating the VEGF Signaling Pathway".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 53.4(2012):1990-1998.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Du, Wei]的文章
[Yu, Wenzhen]的文章
[Huang, Lvzhen]的文章
百度学术
百度学术中相似的文章
[Du, Wei]的文章
[Yu, Wenzhen]的文章
[Huang, Lvzhen]的文章
必应学术
必应学术中相似的文章
[Du, Wei]的文章
[Yu, Wenzhen]的文章
[Huang, Lvzhen]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。