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Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System
Tian Ning1; Zhang Lu2; Zheng Jing-hao1; Li Ying2; Ma Wan-yun1
关键词Two-photon Imaging Quantum Dot Aging In-vitro Maturation Dna Methyltransferase
刊名SPECTROSCOPY AND SPECTRAL ANALYSIS
2012-12-01
DOI10.3964/j.issn.1000-0593(2012)12-3175-04
32期:12页:3175-3178
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Spectroscopy
研究领域[WOS]Spectroscopy
关键词[WOS]IN-VITRO ; MATURATION ; METHYLATION ; NUCLEAR
英文摘要

Quantum dot (QD) is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases (Dnmts) and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors′ results showed that aging mouse oocytes may be not suitable for in-vitro maturation: both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA methylation modification.

语种中文
WOS记录号WOS:000312350900002
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52132
专题基础医学院
作者单位1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Histol & Embryol, Beijing 100191, Peoples R China
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GB/T 7714
Tian Ning,Zhang Lu,Zheng Jing-hao,et al. Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2012,32(12):3175-3178.
APA Tian Ning,Zhang Lu,Zheng Jing-hao,Li Ying,&Ma Wan-yun.(2012).Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System.SPECTROSCOPY AND SPECTRAL ANALYSIS,32(12),3175-3178.
MLA Tian Ning,et al."Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System".SPECTROSCOPY AND SPECTRAL ANALYSIS 32.12(2012):3175-3178.
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