北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus
作者: Qiu, Xiang2; Hong, Chao1,3; Li, Yue2; Bao, Wanrong1,3; Gao, Xiao-Ming1,2,3
关键词: Spike protein ; severe acute respiratory syndrome-associated coronavirus ; calreticulin ; antibody
刊名: MICROBIOLOGY AND IMMUNOLOGY
发表日期: 2012-08-01
DOI: 10.1111/j.1348-0421.2012.00467.x
卷: 56, 期:8, 页:554-561
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Microbiology
研究领域[WOS]: Immunology ; Microbiology
关键词[WOS]: SYSTEMIC-LUPUS-ERYTHEMATOSUS ; TUMOR-SPECIFIC IMMUNITY ; SARS-CORONAVIRUS ; NEUTRALIZING ANTIBODIES ; NUCLEOCAPSID PROTEIN ; RECEPTOR ; FRAGMENT ; ANTIGEN ; DOMAIN ; ANTIANGIOGENESIS
英文摘要:

Fragment 450650 of the spike (S) protein (S450650) of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains epitopes capable of being recognized by convalescent sera of SARS patients. Vaccination of mice with recombinant S450650 (rS450650) can induce Abs against SARS-CoV, although the titer is relatively low. In the present study, a fusion protein linking a fragment (residues 39272) of murine calreticulin (CRT) to S450650 in a prokaryotic expression system was created. Compared with target antigen alone, the recombinant fusion product (rS450650-CRT) has much improved hydrophilicity and immunogenicity. The S450650-specific IgG Abs of BALB/c mice subcutaneously immunized with rS450650-CRT were in substantially higher titer (approximately fivefold more). Furthermore, the fusion protein, but not rS450650 alone, was able to elicit S450650-specific IgG responses in T cell deficient nude mice. Given that rCRT/39272 can drive the maturation of bone-marrow-derived dendritic cells, directly activate macrophages and B cells, and also elicit helper T cell responses in vivo, we propose that fragment 39272 of CRT is an effective molecular adjuvant capable of enhancing target Ag-specific humoral responses in both a T cell-dependent and independent manner. Fusion protein rS450650-CRT is a potential candidate vaccine against SARS-CoV infection.

语种: 英语
所属项目编号: IRT1075 ; 30890142/31070781 ; 2010CB529102
项目资助者: Program for Changjiang Scholars and Innovative Research Team in University ; National Foundation of Natural Science of China ; National Key Basic Research Programs
WOS记录号: WOS:000306896900008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52133
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Qiu_et_al-2012-Microbiology_and_Immunology.pdf(764KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Key Lab Infect & Immun Jiangsu Prov, Suzhou, Peoples R China
2.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China
3.Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Qiu, Xiang,Hong, Chao,Li, Yue,et al. Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus[J]. MICROBIOLOGY AND IMMUNOLOGY,2012,56(8):554-561.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qiu, Xiang]'s Articles
[Hong, Chao]'s Articles
[Li, Yue]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qiu, Xiang]‘s Articles
[Hong, Chao]‘s Articles
[Li, Yue]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace