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学科主题: 基础医学
题名:
Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus
作者: Qiu, Xiang2; Hong, Chao1,3; Li, Yue2; Bao, Wanrong1,3; Gao, Xiao-Ming1,2,3
关键词: Spike protein ; severe acute respiratory syndrome-associated coronavirus ; calreticulin ; antibody
刊名: MICROBIOLOGY AND IMMUNOLOGY
发表日期: 2012-08-01
DOI: 10.1111/j.1348-0421.2012.00467.x
卷: 56, 期:8, 页:554-561
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Microbiology
研究领域[WOS]: Immunology ; Microbiology
关键词[WOS]: SYSTEMIC-LUPUS-ERYTHEMATOSUS ; TUMOR-SPECIFIC IMMUNITY ; SARS-CORONAVIRUS ; NEUTRALIZING ANTIBODIES ; NUCLEOCAPSID PROTEIN ; RECEPTOR ; FRAGMENT ; ANTIGEN ; DOMAIN ; ANTIANGIOGENESIS
英文摘要:

Fragment 450650 of the spike (S) protein (S450650) of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains epitopes capable of being recognized by convalescent sera of SARS patients. Vaccination of mice with recombinant S450650 (rS450650) can induce Abs against SARS-CoV, although the titer is relatively low. In the present study, a fusion protein linking a fragment (residues 39272) of murine calreticulin (CRT) to S450650 in a prokaryotic expression system was created. Compared with target antigen alone, the recombinant fusion product (rS450650-CRT) has much improved hydrophilicity and immunogenicity. The S450650-specific IgG Abs of BALB/c mice subcutaneously immunized with rS450650-CRT were in substantially higher titer (approximately fivefold more). Furthermore, the fusion protein, but not rS450650 alone, was able to elicit S450650-specific IgG responses in T cell deficient nude mice. Given that rCRT/39272 can drive the maturation of bone-marrow-derived dendritic cells, directly activate macrophages and B cells, and also elicit helper T cell responses in vivo, we propose that fragment 39272 of CRT is an effective molecular adjuvant capable of enhancing target Ag-specific humoral responses in both a T cell-dependent and independent manner. Fusion protein rS450650-CRT is a potential candidate vaccine against SARS-CoV infection.

语种: 英语
所属项目编号: IRT1075 ; 30890142/31070781 ; 2010CB529102
项目资助者: Program for Changjiang Scholars and Innovative Research Team in University ; National Foundation of Natural Science of China ; National Key Basic Research Programs
WOS记录号: WOS:000306896900008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52133
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Key Lab Infect & Immun Jiangsu Prov, Suzhou, Peoples R China
2.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China
3.Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Qiu, Xiang,Hong, Chao,Li, Yue,et al. Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus[J]. MICROBIOLOGY AND IMMUNOLOGY,2012,56(8):554-561.
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