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学科主题: 公共卫生
题名:
Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique
作者: Zhou, Hong1,2; Ohno, Nobuhiko1; Terada, Nobuo1; Saitoh, Sei1; Naito, Ichiro3; Ohno, Shinichi1
刊名: REPRODUCTION
发表日期: 2008-11-01
DOI: 10.1530/REP-08-0022
卷: 136, 期:5, 页:599-610
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Reproductive Biology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; CUMULUS EXTRACELLULAR-MATRIX ; FREEZE-SUBSTITUTION ; IV COLLAGEN ; IMMUNOHISTOCHEMICAL ANALYSES ; ANTIPROGESTERONE RU486 ; HEMODYNAMIC CONDITIONS ; RU486-TREATED RATS ; BASEMENT-MEMBRANE ; MEDICAL PROGRESS
英文摘要:

Despite the potential association of polycystic ovary (PCO) syndrome with hemodynamic changes, follicular microenvironment and the involvement of blood follicle barriers (BFB), a histopathological examination has been hampered by artifacts caused by conventional preparation methods. in this study, mouse ovaries of a mifepristone-induced PCO model were morphologically and immunohistochemically examined by in vivo cryotechnique (IVCT), which prevents those technical artifacts. Ovarian specimens of PCO model mice were prepared by IVCT or the conventional perfusion fixation after s.c. injection of mifepristone. Their histology and immunolocalization of plasma proteins, including albumin (molecular mass, 69 kDa), immunoglobulin G (IgG, 150 kDa), inter-alpha-trypsin inhibitor (ITI, 220 kDa), fibrinogen (340 kDa), and IgM (900 kDa), were examined. In the PCO model, enlarged blood vessels with abundant blood flow were observed in addition to cystic follicles with degenerative membrana granulosa. The immunolocalization of albumin and IgM in the PCO model were similar to those in normal mice. Albumin immunolocalized in the blood vessels, interstitium or follicles, and IgM was mostly restricted within the blood vessels. In contrast, immunolocalization of IgG, ITI, and fibrinogen changed in the PCO model. Both IgG and ITI were clearly blocked by follicular basement membranes, and hardly observed in the membrana granulosa, though fibrinogen was mostly observed within blood vessels. These findings suggest that increased blood flow and enhanced selectivity of molecular permeation through the BFB are prominent features in the PCO ovaries, and changes in hemodynamic conditions and permselectivity of BFB are involved in the pathogenesis and pathophysiology of PCO syndrome.

语种: 英语
WOS记录号: WOS:000261000200008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52138
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Anat & Mol Histol, Chuo City, Yamanashi 4093898, Japan
2.Peking Univ, Sch Publ Hlth, Dept Child Adolescent & Womens Hlth, Beijing 100083, Peoples R China
3.Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Human Morphol, Okayama 7008558, Japan

Recommended Citation:
Zhou, Hong,Ohno, Nobuhiko,Terada, Nobuo,et al. Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique[J]. REPRODUCTION,2008,136(5):599-610.
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