IR@PKUHSC  > 北京大学第三临床医学院  > 消化科
学科主题临床医学
The feasibility of light microscopic measurements of intercellular spaces in squamous epithelium in the lower-esophagus of GERD patients
Cui, R.; Zhou, L.; Lin, S.; Xue, Y.; Duan, L.; Xia, Z.; Jin, Z.; Zhang, H.; Zhang, J.; Song, Z.; Yan, X.
关键词Gastroesophageal Reflux Disease Intercellular Space Diameter Light Microscopy Nonerosive Gastroesophageal Reflux Disease Transmission Electronic Microscopy
刊名DISEASES OF THE ESOPHAGUS
2011
DOI10.1111/j.1442-2050.2010.01083.x
24期:1页:1-5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]GASTROESOPHAGEAL-REFLUX DISEASE ; MORPHOLOGICAL FEATURE ; ACID REFLUX ; DISTAL
英文摘要

P>The study aims to determine whether light microscopy can be used to accurately measure the diameters of intercellular spaces between squamous epithelial cells in the lower esophagus, and whether changes in this outcome measure can be used as a diagnostic marker for gastroesophageal reflux disease (GERD). The study has two parts. Part 1 involves 42 asymptomatic controls and 119 patients with typical symptoms of GERD, including 58 with erosive esophagitis (EE), and 61 patients with nonerosive gastroesophageal reflux disease (NERD). All biopsies were taken from the lower esophagus. All samples were observed using an immersion objective, after which diameters were measured by computer-assisted morphometry. Part 2 involves 61 individuals who were randomly selected from part 1, including 19 controls, 13 with NERD and 29 with EE. Diameter measurements using both light microscopy and transmission electron microscopy (TEM) were performed for samples of 61 individuals. Samples from a total of 61 individuals (31 male, 30 female, mean age 44.3 +/- 16.0 years) were observed using both light microscopy and TEM. Both methods showed significant differences between control and disease groups; the outcomes from the two methods had a certain correlation (r = 0.605, P = 0.000). Morphometric analysis of all 161 individuals (83 males, 78 females, mean age 41.4 +/- 15.7) showed mean diameters from light microscopy to be 0.58 +/- 0.16 mu m for controls, 1.07 +/- 0.30 mu m for NERD, and 1.29 +/- 0.20 mu m for EE; differences between control and disease groups were significant (P < 0.05). The optimal cut-off value from receiver operator characteristic analysis was 0.85 mu m. Diagnoses were validated using the combination of symptoms of GERD, endoscopy, and 24 h ambulatory pH monitoring as the gold standard. At the optimal cutoff, sensitivity was 93.3% and specificity was 100%. The diameters of the intercellular spaces in squamous epithelium of lower esophagus from controls and in patients with GERD can be quantitatively measured using light microscopy. Dilated diameters can serve as a sensitive, specific, and objective indicator for diagnosis of GERD.

语种英语
WOS记录号WOS:000286214400002
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52142
Collection北京大学第三临床医学院_消化科
作者单位Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Cui, R.,Zhou, L.,Lin, S.,et al. The feasibility of light microscopic measurements of intercellular spaces in squamous epithelium in the lower-esophagus of GERD patients[J]. DISEASES OF THE ESOPHAGUS,2011,24(1):1-5.
APA Cui, R..,Zhou, L..,Lin, S..,Xue, Y..,Duan, L..,...&Yan, X..(2011).The feasibility of light microscopic measurements of intercellular spaces in squamous epithelium in the lower-esophagus of GERD patients.DISEASES OF THE ESOPHAGUS,24(1),1-5.
MLA Cui, R.,et al."The feasibility of light microscopic measurements of intercellular spaces in squamous epithelium in the lower-esophagus of GERD patients".DISEASES OF THE ESOPHAGUS 24.1(2011):1-5.
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