|Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors|
|Ren, FR1; Lv, QS1; Zhuang, H1; Li, JJ1; Gong, XY1; Gao, GJ1; Liu, CL1; Wang, JX1; Yao, FZ1; Zheng, YR1; Zhu, FM1; Tiemuer, MHL1; Bai, XH1; Shan, H1|
|WOS标题词||Science & Technology|
|关键词[WOS]||ANTIBODIES ; RNA|
BACKGROUND: The correlation between signal-to-cutoff (S/CO) ratios of a second-generation hepatitis C virus (HCV) enzyme immunoassay (EIA; Abbott) and a third-generation HCV enzyme-linked immunosorbent assay (ELISA; Ortho) and confirmed HCV infection has been reported. The utility of the values for the Chinese anti-HCV EIA kits, however, has not been studied in evaluating test results in Chinese blood donors.
STUDY DESIGN AND METHODS: A total of 156 donor samples repeat reactive for anti-HCV at routine screening from five representative regions of China were retested for anti-HCV by the Ortho third-generation HCV ELISA and six Chinese EIA kits and for HCV RNA by a human immunodeficiency virus-1 and HCV assay (Procleix, Chiron Corp.). The HCV RNA-nonreactive samples were further tested for anti-HCV by a third-generation recombinant immunoblot assay RIBA (Chiron Corp.). The positive result by either nucleic acid amplification test or RIBA was interpreted as confirmed HCV infection.
RESULTS: The confirmed HCV prevalence rate in donors in five representative regions obtained in this study was 0.20 percent (77/37,900) in 2004. All seven anti-HCV EIA kits had a significant correlation between S/CO ratios and confirmed HCV infection. The threshold S/CO ratios, which predicted more than 95 percent of confirmed HCV infections for the Ortho, SABC, BGI-GBI, InTec, GWK, KHB, and WANTAI kits, were 3.8, 6.0, 7.0, 8.6, 10.0, 10.0, and 14.0, respectively.
CONCLUSIONS: Anti-HCV EIA kits commonly used in Chinese donors screening demonstrate good correlation between S/CO ratios and the confirmed infection. For the Ortho third-generation HCV ELISA, the S/CO ratio of 3.8 determined by the US Centers for Disease Control and Prevention is applicable to Chinese blood donors. The Chinese domestic EIA kits evaluated show a diverse range of threshold S/CO ratios.
|作者单位||1.Urumchi Blood Ctr Xinjiang Uygur Autonomous Reg, Urumqi, Peoples R China|
2.Xinjiang Prod & Construct Grp, Cent Blood Bank, Urumqi, Peoples R China
3.Beijing Red Cross Blood Ctr, Beijing, Peoples R China
4.Yunnan Kunming Blood Ctr, Kunming, Peoples R China
5.Guangzhou Blood Ctr, Guangzhou, Guangdong, Peoples R China
6.Blood Ctr Zhejiang Prov, Hangzhou, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
8.Chinese Acad Med Sci, Inst Blood Transfus, Chengdu, Peoples R China
9.Johns Hopkins Med Inst, Dept Pathol, Div Blood Transfus, HIV Specialty Testing Lab, Baltimore, MD 21205 USA
|Ren, FR,Lv, QS,Zhuang, H,et al. Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors[J]. TRANSFUSION,2005,45(11):1816-1822.|
|APA||Ren, FR.,Lv, QS.,Zhuang, H.,Li, JJ.,Gong, XY.,...&Shan, H.(2005).Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors.TRANSFUSION,45(11),1816-1822.|
|MLA||Ren, FR,et al."Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors".TRANSFUSION 45.11(2005):1816-1822.|
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