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学科主题: 基础医学
题名:
Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors
作者: Ren, FR1; Lv, QS1; Zhuang, H1; Li, JJ1; Gong, XY1; Gao, GJ1; Liu, CL1; Wang, JX1; Yao, FZ1; Zheng, YR1; Zhu, FM1; Tiemuer, MHL1; Bai, XH1; Shan, H1
刊名: TRANSFUSION
发表日期: 2005-11-01
DOI: 10.1111/j.1537-2995.2005.00611.x
卷: 45, 期:11, 页:1816-1822
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: ANTIBODIES ; RNA
英文摘要:

BACKGROUND: The correlation between signal-to-cutoff (S/CO) ratios of a second-generation hepatitis C virus (HCV) enzyme immunoassay (EIA; Abbott) and a third-generation HCV enzyme-linked immunosorbent assay (ELISA; Ortho) and confirmed HCV infection has been reported. The utility of the values for the Chinese anti-HCV EIA kits, however, has not been studied in evaluating test results in Chinese blood donors.

STUDY DESIGN AND METHODS: A total of 156 donor samples repeat reactive for anti-HCV at routine screening from five representative regions of China were retested for anti-HCV by the Ortho third-generation HCV ELISA and six Chinese EIA kits and for HCV RNA by a human immunodeficiency virus-1 and HCV assay (Procleix, Chiron Corp.). The HCV RNA-nonreactive samples were further tested for anti-HCV by a third-generation recombinant immunoblot assay RIBA (Chiron Corp.). The positive result by either nucleic acid amplification test or RIBA was interpreted as confirmed HCV infection.

RESULTS: The confirmed HCV prevalence rate in donors in five representative regions obtained in this study was 0.20 percent (77/37,900) in 2004. All seven anti-HCV EIA kits had a significant correlation between S/CO ratios and confirmed HCV infection. The threshold S/CO ratios, which predicted more than 95 percent of confirmed HCV infections for the Ortho, SABC, BGI-GBI, InTec, GWK, KHB, and WANTAI kits, were 3.8, 6.0, 7.0, 8.6, 10.0, 10.0, and 14.0, respectively.

CONCLUSIONS: Anti-HCV EIA kits commonly used in Chinese donors screening demonstrate good correlation between S/CO ratios and the confirmed infection. For the Ortho third-generation HCV ELISA, the S/CO ratio of 3.8 determined by the US Centers for Disease Control and Prevention is applicable to Chinese blood donors. The Chinese domestic EIA kits evaluated show a diverse range of threshold S/CO ratios.

语种: 英语
WOS记录号: WOS:000232774700021
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52144
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Urumchi Blood Ctr Xinjiang Uygur Autonomous Reg, Urumqi, Peoples R China
2.Xinjiang Prod & Construct Grp, Cent Blood Bank, Urumqi, Peoples R China
3.Beijing Red Cross Blood Ctr, Beijing, Peoples R China
4.Yunnan Kunming Blood Ctr, Kunming, Peoples R China
5.Guangzhou Blood Ctr, Guangzhou, Guangdong, Peoples R China
6.Blood Ctr Zhejiang Prov, Hangzhou, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
8.Chinese Acad Med Sci, Inst Blood Transfus, Chengdu, Peoples R China
9.Johns Hopkins Med Inst, Dept Pathol, Div Blood Transfus, HIV Specialty Testing Lab, Baltimore, MD 21205 USA

Recommended Citation:
Ren, FR,Lv, QS,Zhuang, H,et al. Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors[J]. TRANSFUSION,2005,45(11):1816-1822.
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