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学科主题基础医学
KLF6/Sp1 initiates transcription of the tmsg-1 gene in human prostate carcinoma cells: An exon involved mechanism
Gong, Miaozi2; Yu, Wenjuan2; Pei, Fei2; You, Jiangfeng2; Cui, Xianglin2; McNutt, Michael A.2; Li, Gang1; Zheng, Jie2
关键词tmsg-1 Tumor suppressor gene Transcriptional regulation Transcription factor Exon
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2012
DOI10.1002/jcb.23359
113期:1页:329-339
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]OPIOID RECEPTOR GENE ; H+-ATPASE ; CERAMIDE ; PROMOTER ; EXPRESSION ; LYMPHOCYTES ; INHIBITION ; LAG1 ; SP1
英文摘要

The tumor metastasis suppressor gene-1 (tmsg-1) was first cloned as a new tumor suppressor gene in our laboratory several years ago. Since then, however, despite the substantial progression that has been made in investigation of the biologic roles played by this gene, the manner in which it exerts its regulatory influence is still unknown. With transfection of various deletion or mutation constructs, we identified a potential enhancer and three potential silencers in the 5′-flanking region. However, it was particularly interesting to find that a region (+59 to +123bp) of exon 1 exhibited a strong role in initiation of tmsg-1 gene transcription. Deletion of this region led to essentially complete loss of driving activity of exon-1 sequence on luciferase. Further analysis showed that transcription factors KLF6 and Sp1 are able to interact with each other and bind to their elements in this region. Co-transfection of pGL3-114/+123 with KLF6- and/or Sp1-expressing plasmids resulted in an elevation of luciferase activity and transcription level of tmsg-1, which was abolished by knockdown of KLF6 or Sp1. Analysis of metastatic capacity showed that cells with high metastatic capability exhibited a lower level of KLF6/TMSG-1 proteins with higher invasive capability and vice versa. Thus, we concluded that interaction of KLF6 and Sp1, together with their binding of elements in exon 1 are critical events in initiation of transcription of the tmsg-1 gene. These results reveal a hitherto unreported mechanism for initiation of transcription of the tmsg-1 gene. J. Cell. Biochem. 113: 329339, 2012. (C) 2011 Wiley Periodicals, Inc.

语种英语
WOS记录号WOS:000298598300036
项目编号30971142 ; 30772536 ; 81072710 ; 7101006 ; 2009CB521805
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China (973 program)
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52149
专题北京大学基础医学院_病理学系
北京大学基础医学院
北京大学医学图书馆_流通阅览部
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
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GB/T 7714
Gong, Miaozi,Yu, Wenjuan,Pei, Fei,et al. KLF6/Sp1 initiates transcription of the tmsg-1 gene in human prostate carcinoma cells: An exon involved mechanism[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2012,113(1):329-339.
APA Gong, Miaozi.,Yu, Wenjuan.,Pei, Fei.,You, Jiangfeng.,Cui, Xianglin.,...&Zheng, Jie.(2012).KLF6/Sp1 initiates transcription of the tmsg-1 gene in human prostate carcinoma cells: An exon involved mechanism.JOURNAL OF CELLULAR BIOCHEMISTRY,113(1),329-339.
MLA Gong, Miaozi,et al."KLF6/Sp1 initiates transcription of the tmsg-1 gene in human prostate carcinoma cells: An exon involved mechanism".JOURNAL OF CELLULAR BIOCHEMISTRY 113.1(2012):329-339.
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