|Role of prostaglandin E-2 and its receptors in the process of ductus arteriosus maturation and functional closure in the rabbit|
|Fan, Fen-Ling3,4; Zhu, Sen1; Chen, Li-Hong1; Zou, Yu-Liang2; Fan, Li-Hong3,4; Kang, Ji-Hong1; Ma, Ai-Qun3,4; Guan, You-Fei1|
|关键词||Fetal Ductus Arteriosus Oxygen Tension Preterm Birth Prostaglandin E-2 Prostanoid Receptors|
|刊名||CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Pharmacology & Pharmacy ; Physiology|
|研究领域[WOS]||Pharmacology & Pharmacy ; Physiology|
|关键词[WOS]||PROSTANOID RECEPTORS ; PRETERM INFANTS ; FETAL ; INDOMETHACIN ; MANAGEMENT ; EXPRESSION ; NEWBORN ; DEATH ; MICE|
P>1. Patent ductus arteriosus (PDA) is a common congenital heart defect in premature infants. The present study was designed to determine the role of the prostaglandin (PG) E-2 pathway in the process of ductus arteriosus (DA) maturation and functional closure.
2. Changes in PGE(2) pathway-related enzymes and receptors in DA in preterm and term rabbits were examined at both the mRNA and protein levels. In addition, responses of DA rings to Po-2 and PGE(2) were determined.
3. Circulating PGE(2) levels remained high until 2 h after birth. High levels of the EP4 receptor were found in preterm DA. These tissues were sensitive to PGE(2), which caused vessel dilation, but were insensitive to increased Po-2. In contrast, DA tissues from term rabbits exhibited an immediate contractile response to increased Po-2 and PGE(2) treatment resulted in vasoconstriction, which was associated with increased EP3 and decreased EP4 receptor expression in term DA.
4. In conclusion, the preterm PDA is maintained by high levels of PGE(2), which mainly binds to the EP4 receptor under conditions of hypoxia. In contrast, in the term DA, in which levels of the EP3 receptor are higher than in preterm DA, exposure to PGE(2) resulted in vasoconstriction under normoxic conditions. These findings suggest that blocking the EP4 receptor may represent a more selective treatment for the preterm PDA, whereas activating the EP3 receptor may be more suitable for the treatment of the term PDA.
|项目编号||30725033 ; 30530340 ; 2010CB912503|
|资助机构||National Natural Science Foundation of China ; National Basic Research Program of China|
|作者单位||1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Ctr Diabet, Beijing 100191, Peoples R China|
2.Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Gynecol & Obstet, Xian 710049, Peoples R China
3.Xi An Jiao Tong Univ, Ctr Cardiovasc Med, Dept Cardiovasc Med, Key Lab Environm & Gene Related Dis,Minist Educ, Xian 710049, Peoples R China
4.Xi An Jiao Tong Univ, Ctr Cardiovasc Med, Res Lab Ion Channelopathy, Key Lab Environm & Gene Related Dis,Minist Educ, Xian 710049, Peoples R China
|Fan, Fen-Ling,Zhu, Sen,Chen, Li-Hong,et al. Role of prostaglandin E-2 and its receptors in the process of ductus arteriosus maturation and functional closure in the rabbit[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2010,37(5-6):574-580.|
|APA||Fan, Fen-Ling.,Zhu, Sen.,Chen, Li-Hong.,Zou, Yu-Liang.,Fan, Li-Hong.,...&Guan, You-Fei.(2010).Role of prostaglandin E-2 and its receptors in the process of ductus arteriosus maturation and functional closure in the rabbit.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,37(5-6),574-580.|
|MLA||Fan, Fen-Ling,et al."Role of prostaglandin E-2 and its receptors in the process of ductus arteriosus maturation and functional closure in the rabbit".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 37.5-6(2010):574-580.|
|j.1440-1681.2010.053（870KB）||期刊论文||作者接受稿||开放获取||CC BY-NC-SA||浏览 请求全文|