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Telmisartan counteracts TGF-beta 1 induced epithelial-to-mesenchymal transition via PPAR-gamma in human proximal tubule epithelial cells
Chen Yumin1; Luo Qiong2; Xiong Zibo2; Liang Wei2; Chen Li2; Xiong Zuying1,2
关键词Ppar-gamma Telmisartan Epithelial-to-mesenchymal Transition Proximal Tubular Epithelial Cells Tgf-beta 1 Gw9662
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2012
5期:6页:522-529
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pathology
研究领域[WOS]Oncology ; Pathology
关键词[WOS]ACTIVATED RECEPTOR-GAMMA ; RENAL FIBROSIS ; ANGIOTENSIN-II ; EXPRESSION ; BINDING ; INJURY ; BETA ; INHIBITION ; TARGET ; LIGAND
英文摘要

Chronic renal failure (CRF) mainly results from kidney fibrosis. Epithelial-to-mesenchymal transition (EMT) occurs in stressed tubular epithelial cells and contributes to renal fibrosis. Transforming growth factor-beta 1 (TGF-beta 1) has been shown to initiate and complete the whole EMT process. Peroxisome proliferators-activated receptor-gamma (PPAR-gamma) exerts anti-inflammatory, anti-fibrotic and vaculo-protective effects on different renal diseases. Telmisartan is a member of angiotensin II (Ang II) receptor blocker (ARB) family. Recent studies show that Telmisartan has a partial agonistic effect on PPAR-gamma. Therefore, we tested the hypothesis that Telmisartan reverses the progression of induced EMT by TGF-beta 1 in cultured human renal proximal tubular epithelial (HK-2) cells. Cultured HK-2 cells were treated with TGF-beta 1 (3 ng/ml), a combination of TGF-beta 1 and Telmisartan (10-200umol/L) and a combination of TGF-beta 1, Telmisartan and GW9662, a PPAR-gamma antagonist for 48 hours. EMT was determined by quantitative real-time PCR analysis of E-cadherin (E-cad), Connective Tissue Growth Factor (CTGF) and PPAR-gamma transcript expression and immunocytochemical analysis of E-cad, alpha-Smooth Muscle Actin (alpha-SMA) and PPAR-gamma protein expression. TGF-beta 1 induced phenotypic EMT in cultured HK-2 cell line via significantly reduced E-cad expression and significantly increased CTGF, alpha-SMA expression in association with the loss of epithelial morphology. Telmisartan reversed all EMT markers in a dose-dependent manner which was inhibited by PPAR antagonist GW9662. In the present study, it was suggested that Telmisartan attenuated TGF-beta 1 induced EMT by agonistic activation of PPAR-gamma.

语种英语
WOS记录号WOS:000308360800005
资助机构Shenzhen Branch Trade Industry and Information Commission
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52169
专题北京大学深圳医院_肾内科
作者单位1.Peking Univ, Shenzhen Hosp, Dept Nephrol, Shenzhen, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Nephrol, Hlth Sci Ctr, Shenzhen, Peoples R China
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GB/T 7714
Chen Yumin,Luo Qiong,Xiong Zibo,et al. Telmisartan counteracts TGF-beta 1 induced epithelial-to-mesenchymal transition via PPAR-gamma in human proximal tubule epithelial cells[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2012,5(6):522-529.
APA Chen Yumin,Luo Qiong,Xiong Zibo,Liang Wei,Chen Li,&Xiong Zuying.(2012).Telmisartan counteracts TGF-beta 1 induced epithelial-to-mesenchymal transition via PPAR-gamma in human proximal tubule epithelial cells.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,5(6),522-529.
MLA Chen Yumin,et al."Telmisartan counteracts TGF-beta 1 induced epithelial-to-mesenchymal transition via PPAR-gamma in human proximal tubule epithelial cells".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 5.6(2012):522-529.
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