北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学深圳医院  > 肾内科  > 期刊论文
学科主题: 临床医学
题名:
Telmisartan counteracts TGF-beta 1 induced epithelial-to-mesenchymal transition via PPAR-gamma in human proximal tubule epithelial cells
作者: Chen Yumin1; Luo Qiong2; Xiong Zibo2; Liang Wei2; Chen Li2; Xiong Zuying1,2
关键词: PPAR-gamma ; telmisartan ; epithelial-to-mesenchymal transition ; proximal tubular epithelial cells ; TGF-beta 1 ; GW9662
刊名: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
发表日期: 2012
卷: 5, 期:6, 页:522-529
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Pathology
研究领域[WOS]: Oncology ; Pathology
关键词[WOS]: ACTIVATED RECEPTOR-GAMMA ; RENAL FIBROSIS ; ANGIOTENSIN-II ; EXPRESSION ; BINDING ; INJURY ; BETA ; INHIBITION ; TARGET ; LIGAND
英文摘要:

Chronic renal failure (CRF) mainly results from kidney fibrosis. Epithelial-to-mesenchymal transition (EMT) occurs in stressed tubular epithelial cells and contributes to renal fibrosis. Transforming growth factor-beta 1 (TGF-beta 1) has been shown to initiate and complete the whole EMT process. Peroxisome proliferators-activated receptor-gamma (PPAR-gamma) exerts anti-inflammatory, anti-fibrotic and vaculo-protective effects on different renal diseases. Telmisartan is a member of angiotensin II (Ang II) receptor blocker (ARB) family. Recent studies show that Telmisartan has a partial agonistic effect on PPAR-gamma. Therefore, we tested the hypothesis that Telmisartan reverses the progression of induced EMT by TGF-beta 1 in cultured human renal proximal tubular epithelial (HK-2) cells. Cultured HK-2 cells were treated with TGF-beta 1 (3 ng/ml), a combination of TGF-beta 1 and Telmisartan (10-200umol/L) and a combination of TGF-beta 1, Telmisartan and GW9662, a PPAR-gamma antagonist for 48 hours. EMT was determined by quantitative real-time PCR analysis of E-cadherin (E-cad), Connective Tissue Growth Factor (CTGF) and PPAR-gamma transcript expression and immunocytochemical analysis of E-cad, alpha-Smooth Muscle Actin (alpha-SMA) and PPAR-gamma protein expression. TGF-beta 1 induced phenotypic EMT in cultured HK-2 cell line via significantly reduced E-cad expression and significantly increased CTGF, alpha-SMA expression in association with the loss of epithelial morphology. Telmisartan reversed all EMT markers in a dose-dependent manner which was inhibited by PPAR antagonist GW9662. In the present study, it was suggested that Telmisartan attenuated TGF-beta 1 induced EMT by agonistic activation of PPAR-gamma.

语种: 英语
项目资助者: Shenzhen Branch Trade Industry and Information Commission
WOS记录号: WOS:000308360800005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52169
Appears in Collections:北京大学深圳医院_肾内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Nephrol, Shenzhen, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Nephrol, Hlth Sci Ctr, Shenzhen, Peoples R China

Recommended Citation:
Chen Yumin,Luo Qiong,Xiong Zibo,et al. Telmisartan counteracts TGF-beta 1 induced epithelial-to-mesenchymal transition via PPAR-gamma in human proximal tubule epithelial cells[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2012,5(6):522-529.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen Yumin]'s Articles
[Luo Qiong]'s Articles
[Xiong Zibo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen Yumin]‘s Articles
[Luo Qiong]‘s Articles
[Xiong Zibo]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace