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学科主题: 基础医学
题名:
Telomerase immortalization of human neural progeniitor cells
作者: Bai, Y1; Hu, QK1; Li, XX1; Wang, YJ1; Lin, CS1; Shen, L1; Li, LS1
关键词: hTERT ; immortalization ; neural progenitor cell line ; telomerase
刊名: NEUROREPORT
发表日期: 2004-02-09
DOI: 10.1097/01.wnr.0000105204.95859.c0
卷: 15, 期:2, 页:245-249
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: HUMAN BONE-MARROW ; IN-VITRO ; PRECURSOR CELLS ; LIFE-SPAN ; EXPRESSION ; EXTENSION ; GROWTH
英文摘要:

It is necessary to expand human neural progenitor cells in vitro to obtain large numbers for research purposes and cell transplantation. A potential obstacle to in vitro expansion, however, is that neural progenitor cells have a limited replication life-span and gradually lose their differentiation potential. We report here that ectopic expression of the catalytic subunit of human telomerase (hTERT) gene in neural progenitor cells could induce telomerase activity, stabilize telomeres and extend their replicative life-spans. The telomerase-immortalized cells (hNPC-TERT) maintained the normal diploid karyotype, expressed the markers of human neural progenitor cells and meanwhile held the differentiation potential in vitro for up to 120 population doublings. This study provides a new approach for obtaining unlimited quantities of normal phenotypic and homogeneous human neural progenitor cells in vitro.

语种: 英语
WOS记录号: WOS:000225139900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52185
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Peking Univ Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Genet, Peking Univ Stem Cell Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Bai, Y,Hu, QK,Li, XX,et al. Telomerase immortalization of human neural progeniitor cells[J]. NEUROREPORT,2004,15(2):245-249.
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