IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
Telomerase immortalization of human neural progeniitor cells
Bai, Y1; Hu, QK1; Li, XX1; Wang, YJ1; Lin, CS1; Shen, L1; Li, LS1
关键词Htert Immortalization Neural Progenitor Cell Line Telomerase
刊名NEUROREPORT
2004-02-09
DOI10.1097/01.wnr.0000105204.95859.c0
15期:2页:245-249
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]HUMAN BONE-MARROW ; IN-VITRO ; PRECURSOR CELLS ; LIFE-SPAN ; EXPRESSION ; EXTENSION ; GROWTH
英文摘要

It is necessary to expand human neural progenitor cells in vitro to obtain large numbers for research purposes and cell transplantation. A potential obstacle to in vitro expansion, however, is that neural progenitor cells have a limited replication life-span and gradually lose their differentiation potential. We report here that ectopic expression of the catalytic subunit of human telomerase (hTERT) gene in neural progenitor cells could induce telomerase activity, stabilize telomeres and extend their replicative life-spans. The telomerase-immortalized cells (hNPC-TERT) maintained the normal diploid karyotype, expressed the markers of human neural progenitor cells and meanwhile held the differentiation potential in vitro for up to 120 population doublings. This study provides a new approach for obtaining unlimited quantities of normal phenotypic and homogeneous human neural progenitor cells in vitro.

语种英语
WOS记录号WOS:000225139900006
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52185
专题北京大学基础医学院_细胞生物学系
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Peking Univ Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Genet, Peking Univ Stem Cell Res Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Bai, Y,Hu, QK,Li, XX,et al. Telomerase immortalization of human neural progeniitor cells[J]. NEUROREPORT,2004,15(2):245-249.
APA Bai, Y.,Hu, QK.,Li, XX.,Wang, YJ.,Lin, CS.,...&Li, LS.(2004).Telomerase immortalization of human neural progeniitor cells.NEUROREPORT,15(2),245-249.
MLA Bai, Y,et al."Telomerase immortalization of human neural progeniitor cells".NEUROREPORT 15.2(2004):245-249.
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