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学科主题: 药学
题名:
LC analysis and pharmacokinetic study of pachymic acid after intravenous administration to rats
作者: Alatengqimuge, Yue1; Yang, Xiu-Wei1; Zheng, Yan1; Ma, Lian1; Lu, Wei2
关键词: column liquid chromatography ; pharmacokinetics ; rat plasma ; pachymic acid
刊名: CHROMATOGRAPHIA
发表日期: 2008-05-01
DOI: 10.1365/s10337-008-0577-7
卷: 67, 期:9-10, 页:807-811
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PORIA-COCOS ; CONSTITUENTS ; CELLS
英文摘要:

A simple and sensitive high-performance liquid chromatographic method with UV detection was developed and validated to investigate the concentration of pachymic acid (PA) in rat plasma. The sample preparation was a liquid-liquid extraction and chromatographic separation was achieved with a Dikma Diamonsil (TM) C(18) column (250 x 4.6 mm I.D.) with a C(18) guard column (8 x 4 mm I.D.) using a mobile phase consisting of MeOH-MeCN-aq. 0.45% H(3)PO(4) (45:40:22) at a flow rate of 1.0 mL min(-1). The UV detection was at 210 nm. Standard curves were linear (r = 0.9998) in plasma over the concentration range of 0.5-50 mu g mL(-1) and had acceptable accuracy and precision. Intra- and inter-day precisions expressed as the relative standard deviation (RSD) were 0.26-1.60% and 1.24-2.31%. The lower limit of quantification and lower limit of detection were 0.45 and 0.17 mu g mL(-1). The method has been used successfully to study the pharmacokinetics of PA. After a dose of 30 mg kg(-1) by intravenous administration, the main pharmacokinetic parameters t(1/2), AUC(0-infinity), CL, V(ss) and MRT(0-infinity) were 8.79 +/- 6.80 h, 18.90 +/- 9.39 mu g h mL(-1), 0.53 +/- 0.28 L h(-1), 5.60 +/- 4.60 L and 12.58 +/- 9.95 h, respectively.

语种: 英语
WOS记录号: WOS:000255751100022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52194
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Dept Pharmaceut, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Alatengqimuge, Yue,Yang, Xiu-Wei,Zheng, Yan,et al. LC analysis and pharmacokinetic study of pachymic acid after intravenous administration to rats[J]. CHROMATOGRAPHIA,2008,67(9-10):807-811.
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