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学科主题临床医学
AngRem104, an angiotensin II-induced novel upregulated gene in human mesangial cells, is potentially involved in the regulation of fibronectin expression
Liang, XB; Zhang, H; Zhou, AY; Wang, HY
刊名JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
2003-06-01
DOI10.1097/01.ASN.000056860.64692.C0
14期:6页:1443-1451
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]GREEN FLUORESCENT PROTEIN ; GROWTH-FACTOR-BETA ; MATRIX PROTEINS ; MESSENGER-RNA ; BIOSYNTHESIS ; COLLAGEN ; BINDING ; SYSTEM ; PROLIFERATION ; PROGRESSION
英文摘要

Accumulation of extracellular matrix (ECM) in the glomerular mesangium is a common feature of many progressive renal diseases. Angiotensin II (AngII) plays important roles in the proliferation of glomerular mesangial cells (MC) as well as the synthesis of ECM such as fibronectin (FN) and collagens. However, the precise molecular signals responsible for these effects are unknown. To explore possible molecule mechanism of ECM accumulation related to AngII, suppression subtractive hybridization (SSH) was performed to screen and identify upregulated genes induced by AngII in cultured human MC. A novel gene, AngRem104 (GenBank accession number, AF367870), was isolated. The full-length cDNA of AngRem104 is 1690 bp, and it contains a 1041-bp open reading frame (ORF) encoding 347 amino acid residues with a predicted molecular mass of 37.2 kD. AngRem104 widely expressed in human heart, placenta, liver, muscle, kidney, and pancreas. Moreover, AngRem104 was found in human glomeruli and tubule by in situ hybridization. In human MC, the upregulation of AngRem104 induced by AngII was time-dependent, and it was dose-dependently blocked by AngII type I receptor antagonist (AT1RA), Losartan. The subcellular localization detected by AngRem104-pEGFP fusion protein revealed that AngRem104 was a nuclear protein. Interestingly, when AngRem104 was overexpressed by transfection of its sense construct, cDNA Microarray showed that two of the ECM-related genes, i.e., human mRNA for FN and integrin-beta-1 (FN receptor), dramatically upregulated their expressions. Furthermore, AngRem104 could regulate the expression of FN induced by AngII, which were detected by RT-PCR and quantitative real-time PCR, when AngRem104 was overexpressed. It is concluded that AngRem104 is a novel human gene potentially involved in the regulation of FN induced by AngII in human MC. These findings may provide new insights into mechanisms of glomerular sclerosis associated with AngII.

语种英语
WOS记录号WOS:000183095600004
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52207
专题北京大学第一临床医学院_肾脏内科
作者单位Peking Univ, Div Renal, Hosp 1, Inst Nephrol, Beijing 100034, Peoples R China
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Liang, XB,Zhang, H,Zhou, AY,et al. AngRem104, an angiotensin II-induced novel upregulated gene in human mesangial cells, is potentially involved in the regulation of fibronectin expression[J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,2003,14(6):1443-1451.
APA Liang, XB,Zhang, H,Zhou, AY,&Wang, HY.(2003).AngRem104, an angiotensin II-induced novel upregulated gene in human mesangial cells, is potentially involved in the regulation of fibronectin expression.JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,14(6),1443-1451.
MLA Liang, XB,et al."AngRem104, an angiotensin II-induced novel upregulated gene in human mesangial cells, is potentially involved in the regulation of fibronectin expression".JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 14.6(2003):1443-1451.
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