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Comparative study of laser-induced choroidal neovascularization in rats by paraffin sections, frozen sections and high-resolution optical coherence tomography
Jiao, Jian1; Mo, Bin2; Wei, Hang1; Jiang, Yan-rong1
关键词Paraffin Section Frozen Section Optical Coherence Tomography Choroidal Neovascularization Rat
刊名GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
2013
DOI10.1007/s00417-012-2204-4
251期:1页:301-307
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
资助者Research Fund for the Doctoral Program of Higher Education of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Novel Program ; Research Fund for the Doctoral Program of Higher Education of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Novel Program
研究领域[WOS]Ophthalmology
关键词[WOS]MODEL ; MORPHOLOGY ; HISTOLOGY ; RETINA ; MICE ; AGE
英文摘要

The purpose of this study was to demonstrate the value of a quantitative study concerning experimental choroidal neovascularization (CNV) tissue by correlating the paraffin sections and frozen sections with optical coherence tomography (OCT) images.

After OCT imaging was performed, four right eyes from the normal Brown Norway (BN) rats were made into paraffin sections and four left eyes were made into frozen sections. Laser photocoagulation was performed on the right eyes of the other 32 BN rats to induce CNV; these rats then were divided into two groups. After the OCT imaging was performed, four eyes were made into serial paraffin sections in Group A or frozen sections in Group B at 1 week after laser photocoagulation, and weekly for 4 weeks. A quantitative comparison of histopathological images and OCT images was performed.

The thickness of the sensory retina and choroid of normal BN rats in paraffin sections was perceptibly less than that in the frozen sections, and the thickness of the sensory retina in the frozen sections was similar to that measured in the OCT images. The maximum thickness of the CNV tissue in the frozen sections was greater than that in the paraffin sections, but similar to that measured in the OCT images from week 1 to week 4.

Only the frozen sections accurately matched the high-resolution OCT images because of the processing artifacts in the paraffin sections. In further quantitative studies on experimental CNV, the frozen sections may be more preferable if the thickness of the CNV tissue is to be measured and compared.

语种英语
所属项目编号20100001110073 ; 20110001110042 ; 7112142 ; 30901639 ; 009B04
资助者Research Fund for the Doctoral Program of Higher Education of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Novel Program ; Research Fund for the Doctoral Program of Higher Education of China ; Beijing Natural Science Foundation ; National Natural Science Foundation of China ; Beijing Novel Program
WOS记录号WOS:000313074100035
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52210
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Minist Educ, Dept Ophthalmol, Key Lab Vis Loss & Restorat,Peoples Hosp, Beijing 100044, Peoples R China
2.Capital Med Univ, Beijing Tongren Eye Ctr, Beijing Tongren Hosp, Beijing Ophthalmol & Visual Sci Key Lab, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Jiao, Jian,Mo, Bin,Wei, Hang,et al. Comparative study of laser-induced choroidal neovascularization in rats by paraffin sections, frozen sections and high-resolution optical coherence tomography[J]. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,2013,251(1):301-307.
APA Jiao, Jian,Mo, Bin,Wei, Hang,&Jiang, Yan-rong.(2013).Comparative study of laser-induced choroidal neovascularization in rats by paraffin sections, frozen sections and high-resolution optical coherence tomography.GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,251(1),301-307.
MLA Jiao, Jian,et al."Comparative study of laser-induced choroidal neovascularization in rats by paraffin sections, frozen sections and high-resolution optical coherence tomography".GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY 251.1(2013):301-307.
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