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学科主题临床医学
In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation
Xin Ying1; Zhang ZiPin2; Yu Ping2; Ma FuRong1; Mao LanQun2
关键词On-line Electrochemical Detection In Vivo Microdialysis Magnesium Vertigo
刊名SCIENCE CHINA-CHEMISTRY
2013-02-01
DOI10.1007/s11426-012-4567-0
56期:2页:256-261
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]NEUROTOXICITY ; DIZZINESS ; GLUTAMATE ; NEURONS ; MICRODIALYSIS ; EPIDEMIOLOGY ; RELEASE ; UPDATE ; CA2+
英文摘要

Vertigo is one of the most common clinical symptoms. However, the chemical processes involved in the pathological mechanism of vertigo remain to be fully understood. In this study, we investigate the dynamic changes in the magnesium (Mg2+) concentration in medial vestibular nucleus (MVN) of guinea pigs following vertigo induced by vestibular ice water stimulation with an electrochemical detection method consisting of in vivo microdialysis and on-line selective electrochemical detection. Electrochemical detection of Mg2+ was accomplished based on the current enhancement of Mg2+ towards the electrocatalytic oxidation of NADH at the electrodes modified with the polymerized film of toluidine blue O (TBO). Selectivity for the on-line electrochemical detection against Ca2+ was achieved by using ethyleneglcol-bis(2-aminoethylether) tetraacetic acid (EGTA) as the selective masking agent for Ca2+. The basal level of the extracellular Mg2+ in the MVN of guinea pigs was determined to be 759.7 +/- 176.2 mu M(n = 16). Upon ice water irrigation of the left external ear canal, the concentration of Mg2+ in the MVN decreases significantly, reaches 72 +/- 6% (n = 8) of the basal level, and maintains for at least 1000 s. Control experiments reveal that neither warm water irrigation of the external ear canal nor ice water irrigation of the auricle induces the decrease in the concentration of Mg2+ in the MVN. These results demonstrate that the extracellular Mg2+ in the MVN decreases significantly following vertigo induced by vestibular ice water stimulation. This demonstration suggests that Mg2+ might play an important role in the pathological mechanism of vertigo.

语种英语
WOS记录号WOS:000314271900012
项目编号20975104 ; 20935005 ; 90813032 ; 21127901 ; 91132708 ; 7082100
资助机构National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52213
专题北京大学第三临床医学院_耳鼻喉科
作者单位1.Peking Univ, Hosp 3, Dept Otorhinolaryngol, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
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GB/T 7714
Xin Ying,Zhang ZiPin,Yu Ping,et al. In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation[J]. SCIENCE CHINA-CHEMISTRY,2013,56(2):256-261.
APA Xin Ying,Zhang ZiPin,Yu Ping,Ma FuRong,&Mao LanQun.(2013).In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation.SCIENCE CHINA-CHEMISTRY,56(2),256-261.
MLA Xin Ying,et al."In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation".SCIENCE CHINA-CHEMISTRY 56.2(2013):256-261.
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