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学科主题临床医学
Adenovirus-mediated herpes simplex virus thymidine kinase gene transfer driver by KDR promoter in treatment of experimental human hepatocelLular carcinoma in nude mice
Li Bao-jin1,2,3; Zhang Chao1,2; Yi Yuan-xue1,2; Hao Ying1,2; Liu Xiao-ping1,2; Ou Qing-jia3
关键词Hepatocellular Carcinoma Kdr Promoter Simpler Virus Thymidine Kinase Adenovirus Vector
刊名CHINESE JOURNAL OF CANCER RESEARCH
2007-03-01
DOI10.1007/s11670-007-0022-8
19期:1页:22-26
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; SUICIDE GENE ; IN-VIVO ; TUMOR ; EXPRESSION ; THERAPY ; CONSTRUCTION ; CHEMOTHERAPY ; TARGET ; CANCER
英文摘要

Objective: To investigate the therapeutic efficacy of adenovirus- mediated herpes simplex virus thymidine kinase ( HSV- tk) gene transfer under the driving of KDR promoter ( AdKDR- tk) in combination of ganciclovir ( GCV) against human hepatocellular carcinoma in nude mice. Methods: HepG2 cell line was implanted subcutaneously into 32 nude mice, which were subsequently divided into 4 groups ( n= 8 each group): Ganciclovir group ( I), Ad group ( II), AdCMV- tk/ GCV group ( under the driving of CMV promoter) ( III) and AdKDR- tk/ GCV group ( IV). Then intratumoral injection of recombinant adenovirus or Ad was performed in all nude mice, and repeated 24 h later. For the following 10 d GCV was given at a dose of 100 mg/( kg center dot d), ip. All the treated animals were killed to evaluate the tumor weight and the histopathological changes and the microvessel density of tumors after the treatment was determined. Results: Compared with group I, the tumor inhibitory rate was 12.3% in group III and 24.5% in group IV; the inhibition rates were significantly different between group III and IV ( P < 0.05). The mean MVDs in group I, II, III and IV were 37.4 +/- 8.6, 30.6 +/- 7.8, 27.6 +/- 7.1, and 10.7 +/- 4.1 ( microvessels/ mm(2)), respectively. Significant differences were found between group III and II ( P < 0.05), IV and II ( P < 0.01), and IV and III ( P < 0.01). Conclusion: Intratumoral injection of AdKDR- tk results in marked inhibition of HCC growth through inhibition angiogenesis in nude mice. It may be a new treatment approach for human HCC.

语种英语
WOS记录号WOS:000254850700005
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52221
专题北京大学深圳医院
北京大学第二临床医学院_产科
作者单位1.Peking Univ, Shenzhen Hosp, Dept Hepatobiliary & Laparoscop Surg, Shenzhen 518036, Peoples R China
2.Hong Kong Univ Sci & Technol, Shenzhen Med Ctr, Shenzhen 518036, Peoples R China
3.Sun Yat Sen Univ, Dept Hepatobiliary Surg, Second Affiliated Hosp, Guangzhou 510120, Peoples R China
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GB/T 7714
Li Bao-jin,Zhang Chao,Yi Yuan-xue,et al. Adenovirus-mediated herpes simplex virus thymidine kinase gene transfer driver by KDR promoter in treatment of experimental human hepatocelLular carcinoma in nude mice[J]. CHINESE JOURNAL OF CANCER RESEARCH,2007,19(1):22-26.
APA Li Bao-jin,Zhang Chao,Yi Yuan-xue,Hao Ying,Liu Xiao-ping,&Ou Qing-jia.(2007).Adenovirus-mediated herpes simplex virus thymidine kinase gene transfer driver by KDR promoter in treatment of experimental human hepatocelLular carcinoma in nude mice.CHINESE JOURNAL OF CANCER RESEARCH,19(1),22-26.
MLA Li Bao-jin,et al."Adenovirus-mediated herpes simplex virus thymidine kinase gene transfer driver by KDR promoter in treatment of experimental human hepatocelLular carcinoma in nude mice".CHINESE JOURNAL OF CANCER RESEARCH 19.1(2007):22-26.
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