IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
Detection of EWSR1 translocation with nuclear extraction-based fluorescence in situ hybridization for diagnosis of Ewing′s Sarcoma/Primitive neuroectodermal tumor
Yang, Yu1; Zhang, Lanjing1; Wei, Yanyu1; Wang, Hua1; Xiong, Wei1; Chen, Zheng1; Hes, Ondrej1; Zheng, Jie1
关键词ewing&prime s sarcoma/primitive neuroectodermal tumor fluorescence in situ hybridization reverse transcription polymerase chain reaction sarcoma Ewing&prime s
刊名ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
2007-08-01
29期:4页:221-230
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]PARAFFIN-EMBEDDED TISSUE ; ROUND-CELL TUMORS ; RT-PCR ; MOLECULAR DIAGNOSIS ; SARCOMA ; FUSION ; GENE ; TRANSCRIPTS ; PROTEIN ; FAMILY
英文摘要

Objective: To investigate nuclear extraction-based fluorescence in situ hybridization (NE-FISH) in EWSR1 translocation of Ewing′s sarcoma/primitive neuroectodermal tumor (ES/PNET) in formalin-fixed, paraffin-embedded (FFPE) tissue, compared to thin-section FISH (TS-FISH) and nested reverse-transcription polymerase chain reaction (RT-PCR).

Study design: Nuclei of the tumor cells and total ribonucleic acid (RNA) were extracted from 10 cases of ES/PNET. NE-FISH and TS-FISH were used to analyze the EWS gene translocation. RT-PCR was used to detect t(11;22)(q24;q12) and t(21;22)(q22;q12) fusion transcripts.

Results: Of 10 cases, 9 (90%) were abnormal by NE-FISH and TS-FISH. The average rate of abnormal split signals detected by NE-FISH was significantly higher than by TS-FISH. We analyzed 10 ES/PNET cases and 8 various normal human tissues by NE-FISH and nested RT-PCR. Fusion signals were found in 9 (90%) ES/PNET cases by NE-FISH and 8 (80%) by nested RTPCR. Of 8 normal tissues, none was abnormal by either FISH or RT-PCR.

Conclusion: NE-FISH is more reliable than TS-FISH in detecting EWSR1 translocation of ES/PNET in FFPE tissue, although the FISH techniques were concordant. Nested RT-PCR could be more sensitive than conventional RT-PCR. A combination of NE-FISH and nested RT-PCR would improve accuracy of molecular diagnosis of ES/PNET.

语种英语
WOS记录号WOS:000248821800005
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52228
Collection北京大学基础医学院_病理学系
作者单位1.Mt Sinai Sch Med, Dept Pathol, New York, NY USA
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
3.Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
4.Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA
5.Univ British Columbia, Brain Res Ctr, Vancouver, BC V5Z 1M9, Canada
6.Charles Univ Prague, Med Fac Hosp, Dept Pathol, Plzen, Czech Republic
Recommended Citation
GB/T 7714
Yang, Yu,Zhang, Lanjing,Wei, Yanyu,等. Detection of EWSR1 translocation with nuclear extraction-based fluorescence in situ hybridization for diagnosis of Ewing′s Sarcoma/Primitive neuroectodermal tumor[J]. ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY,2007,29(4):221-230.
APA Yang, Yu.,Zhang, Lanjing.,Wei, Yanyu.,Wang, Hua.,Xiong, Wei.,...&Zheng, Jie.(2007).Detection of EWSR1 translocation with nuclear extraction-based fluorescence in situ hybridization for diagnosis of Ewing′s Sarcoma/Primitive neuroectodermal tumor.ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY,29(4),221-230.
MLA Yang, Yu,et al."Detection of EWSR1 translocation with nuclear extraction-based fluorescence in situ hybridization for diagnosis of Ewing′s Sarcoma/Primitive neuroectodermal tumor".ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY 29.4(2007):221-230.
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