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学科主题: 基础医学
题名:
Detection of EWSR1 translocation with nuclear extraction-based fluorescence in situ hybridization for diagnosis of Ewing′s Sarcoma/Primitive neuroectodermal tumor
作者: Yang, Yu1; Zhang, Lanjing1; Wei, Yanyu1; Wang, Hua1; Xiong, Wei1; Chen, Zheng1; Hes, Ondrej1; Zheng, Jie1
关键词: ewing&prime ; s sarcoma/primitive neuroectodermal tumor ; fluorescence in situ hybridization ; reverse transcription polymerase chain reaction ; sarcoma ; Ewing&prime ; s
刊名: ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
发表日期: 2007-08-01
卷: 29, 期:4, 页:221-230
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: PARAFFIN-EMBEDDED TISSUE ; ROUND-CELL TUMORS ; RT-PCR ; MOLECULAR DIAGNOSIS ; SARCOMA ; FUSION ; GENE ; TRANSCRIPTS ; PROTEIN ; FAMILY
英文摘要:

Objective: To investigate nuclear extraction-based fluorescence in situ hybridization (NE-FISH) in EWSR1 translocation of Ewing′s sarcoma/primitive neuroectodermal tumor (ES/PNET) in formalin-fixed, paraffin-embedded (FFPE) tissue, compared to thin-section FISH (TS-FISH) and nested reverse-transcription polymerase chain reaction (RT-PCR).

Study design: Nuclei of the tumor cells and total ribonucleic acid (RNA) were extracted from 10 cases of ES/PNET. NE-FISH and TS-FISH were used to analyze the EWS gene translocation. RT-PCR was used to detect t(11;22)(q24;q12) and t(21;22)(q22;q12) fusion transcripts.

Results: Of 10 cases, 9 (90%) were abnormal by NE-FISH and TS-FISH. The average rate of abnormal split signals detected by NE-FISH was significantly higher than by TS-FISH. We analyzed 10 ES/PNET cases and 8 various normal human tissues by NE-FISH and nested RT-PCR. Fusion signals were found in 9 (90%) ES/PNET cases by NE-FISH and 8 (80%) by nested RTPCR. Of 8 normal tissues, none was abnormal by either FISH or RT-PCR.

Conclusion: NE-FISH is more reliable than TS-FISH in detecting EWSR1 translocation of ES/PNET in FFPE tissue, although the FISH techniques were concordant. Nested RT-PCR could be more sensitive than conventional RT-PCR. A combination of NE-FISH and nested RT-PCR would improve accuracy of molecular diagnosis of ES/PNET.

语种: 英语
WOS记录号: WOS:000248821800005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52228
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Mt Sinai Sch Med, Dept Pathol, New York, NY USA
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
3.Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
4.Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA
5.Univ British Columbia, Brain Res Ctr, Vancouver, BC V5Z 1M9, Canada
6.Charles Univ Prague, Med Fac Hosp, Dept Pathol, Plzen, Czech Republic

Recommended Citation:
Yang, Yu,Zhang, Lanjing,Wei, Yanyu,et al. Detection of EWSR1 translocation with nuclear extraction-based fluorescence in situ hybridization for diagnosis of Ewing′s Sarcoma/Primitive neuroectodermal tumor[J]. ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY,2007,29(4):221-230.
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