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IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study
作者: Wang, He1; Xiao, Meng1; Kong, Fanrong2; Chen, Sharon2; Dou, Hong-Tao1; Sorrell, Tania2; Li, Ruo-Yu3; Xu, Ying-Chun1
刊名: JOURNAL OF CLINICAL MICROBIOLOGY
发表日期: 2011-05-01
DOI: 10.1128/JCM.02415-10
卷: 49, 期:5, 页:1890-1898
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: MOLECULAR PHYLOGENETIC DIVERSITY ; IMMUNOCOMPROMISED PATIENTS ; OXYSPORUM COMPLEX ; CONTACT-LENS ; INFECTIONS ; ASPERGILLUS ; RESISTANCE ; FUNGI ; SPP. ; DIAGNOSIS
英文摘要:

Eleven reference and 25 clinical isolates of Fusarium were subject to multilocus DNA sequence analysis to determine the species and haplotypes of the fusarial isolates from Beijing and Shandong, China. Seven loci were analyzed: the translation elongation factor 1 alpha gene (EF-1 alpha); the nuclear rRNA internal transcribed spacer (ITS), large subunit (LSU), and intergenic spacer (IGS) regions; the second largest subunit of the RNA polymerase gene (RPB2); the calmodulin gene (CAM); and the mitochondrial small subunit (mtSSU) rRNA gene. We also evaluated an IGS-targeted PCR/reverse line blot (RLB) assay for species/haplotype identification of Fusarium. Twenty Fusarium species and seven species complexes were identified. Of 25 clinical isolates (10 species), the Gibberella (Fusarium) fujikuroi species complex was the commonest (40%) and was followed by the Fusarium solani species complex (FSSC) (36%) and the F. incarnatum-F. equiseti species complex (12%). Six FSSC isolates were identified to the species level as FSSC-3+4, and three as FSSC-5. Twenty-nine IGS, 27 EF-1 alpha, 26 RPB2, 24 CAM, 18 ITS, 19 LSU, and 18 mtSSU haplotypes were identified; 29 were unique, and haplotypes for 24 clinical strains were novel. By parsimony informative character analysis, the IGS locus was the most phylogenetically informative, and the rRNA gene regions were the least. Results by RLB were concordant with multilocus sequence analysis for all isolates. Amphotericin B was the most active drug against all species. Voriconazole MICs were high (>8 mu g/ml) for 15 (42%) isolates, including FSSC. Analysis of larger numbers of isolates is required to determine the clinical utility of the seven-locus sequence analysis and RLB assay in species classification of fusaria.

语种: 英语
所属项目编号: 200802026
项目资助者: Ministry of Health of the People&prime ; s Republic of China, public service sector
WOS记录号: WOS:000289941000028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52239
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Clin Lab, Beijing 100730, Peoples R China
2.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
3.Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing 100034, Peoples R China

Recommended Citation:
Wang, He,Xiao, Meng,Kong, Fanrong,et al. Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2011,49(5):1890-1898.
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