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学科主题临床医学
Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study
Wang, He1; Xiao, Meng1; Kong, Fanrong2; Chen, Sharon2; Dou, Hong-Tao1; Sorrell, Tania2; Li, Ruo-Yu3; Xu, Ying-Chun1
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2011-05-01
DOI10.1128/JCM.02415-10
49期:5页:1890-1898
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]MOLECULAR PHYLOGENETIC DIVERSITY ; IMMUNOCOMPROMISED PATIENTS ; OXYSPORUM COMPLEX ; CONTACT-LENS ; INFECTIONS ; ASPERGILLUS ; RESISTANCE ; FUNGI ; SPP. ; DIAGNOSIS
英文摘要

Eleven reference and 25 clinical isolates of Fusarium were subject to multilocus DNA sequence analysis to determine the species and haplotypes of the fusarial isolates from Beijing and Shandong, China. Seven loci were analyzed: the translation elongation factor 1 alpha gene (EF-1 alpha); the nuclear rRNA internal transcribed spacer (ITS), large subunit (LSU), and intergenic spacer (IGS) regions; the second largest subunit of the RNA polymerase gene (RPB2); the calmodulin gene (CAM); and the mitochondrial small subunit (mtSSU) rRNA gene. We also evaluated an IGS-targeted PCR/reverse line blot (RLB) assay for species/haplotype identification of Fusarium. Twenty Fusarium species and seven species complexes were identified. Of 25 clinical isolates (10 species), the Gibberella (Fusarium) fujikuroi species complex was the commonest (40%) and was followed by the Fusarium solani species complex (FSSC) (36%) and the F. incarnatum-F. equiseti species complex (12%). Six FSSC isolates were identified to the species level as FSSC-3+4, and three as FSSC-5. Twenty-nine IGS, 27 EF-1 alpha, 26 RPB2, 24 CAM, 18 ITS, 19 LSU, and 18 mtSSU haplotypes were identified; 29 were unique, and haplotypes for 24 clinical strains were novel. By parsimony informative character analysis, the IGS locus was the most phylogenetically informative, and the rRNA gene regions were the least. Results by RLB were concordant with multilocus sequence analysis for all isolates. Amphotericin B was the most active drug against all species. Voriconazole MICs were high (>8 mu g/ml) for 15 (42%) isolates, including FSSC. Analysis of larger numbers of isolates is required to determine the clinical utility of the seven-locus sequence analysis and RLB assay in species classification of fusaria.

语种英语
WOS记录号WOS:000289941000028
项目编号200802026
资助机构Ministry of Health of the People&prime ; s Republic of China, public service sector
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52239
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Clin Lab, Beijing 100730, Peoples R China
2.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
3.Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Wang, He,Xiao, Meng,Kong, Fanrong,et al. Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2011,49(5):1890-1898.
APA Wang, He.,Xiao, Meng.,Kong, Fanrong.,Chen, Sharon.,Dou, Hong-Tao.,...&Xu, Ying-Chun.(2011).Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study.JOURNAL OF CLINICAL MICROBIOLOGY,49(5),1890-1898.
MLA Wang, He,et al."Accurate and Practical Identification of 20 Fusarium Species by Seven-Locus Sequence Analysis and Reverse Line Blot Hybridization, and an In Vitro Antifungal Susceptibility Study".JOURNAL OF CLINICAL MICROBIOLOGY 49.5(2011):1890-1898.
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