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Fibroblasts isolated from a keratocystic odontogenic tumor promote osteoclastogenesis in vitro via interaction with epithelial cells
Wang, H-C; Jiang, W-P; Sima, Z-H; Li, T-J
关键词Epithelial-mesenchymal Interaction Fibroblast Kcot Osteoclastogenesis Rankl/opg
刊名ORAL DISEASES
2015-03-01
DOI10.1111/odi.12231
21期:2页:170-177
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]PROSTATE-CANCER ; EXPRESSION ; CYSTS ; BONE ; STROMA ; GENE ; PROSTAGLANDIN-E-2 ; AMELOBLASTOMAS ; PROLIFERATION ; INTERLEUKIN-6
英文摘要

OBJECTIVES: Investigate the role of the epithelial-mesenchymal interaction of keratocystic odontogenic tumor (KCOT) in influencing osteoclastogenesis.

MATERIALS AND METHODS: Fibroblasts isolated from KCOT fibrous capsule and normal gingival mucosa were, respectively, co-cultured with human immortalized oral epithelial cells (HIOECs), and the supernatant was collected to make conditioned medium, in which the osteoclastogenesis of osteoclast precursor cell line Raw 264.7 was observed. Genes related to bone resorption (RANKL, OPG, COX-2, and M-CSF) were analyzed by real-time PCR. Antibodies against human sRANKL and inhibitor of COX-2: NS398 were added to conditioned medium to investigate the inhibitory effect on osteoclastogenesis.

RESULTS: Compared with co-cultured gingival fibroblasts and HIOECs (GE-CM), the conditioned medium from co-cultured KCOT fibroblasts and HIOECs (KE-CM) induced more osteoclast-like cell formation and increased NFATC1 mRNA in Raw264.7 cells (P < 0.05). Co-cultured KCOT fibroblasts (KF) and HIOECs, respectively, expressed more COX-2 mRNA than the co-cultured gingival fibroblasts (GF) and HIOECs (P < 0.05). While the ratio of RANKL/OPG in HIOECs co-cultured with KF was also significantly higher than that co-cultured with GF (P < 0.05). The anti-human sRANKL antibody in KE-CM inhibited osteoclastogenesis of Raw264.7 cells; however, NS398 displayed little inhibition.

CONCLUSION: An interesting phenomenon of osteoclastogenic effect of KE-CM in vitro was investigated, which suggested an indispensable role of epithelial-mesenchymal interaction of KCOT in its bone destruction. It could be at least partly attributed to the up-regulated ratio of RANKL/OPG in epithelium induced by KCOT fibroblasts, the aggressiveness of tumor as result of epithelial-mesenchymal interaction deserves exploration further.

语种英语
所属项目编号81030018 ; 30872900 ; 20120001110043
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China
WOS记录号WOS:000349986500006
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52256
专题北京大学口腔医学院_口腔病理科
作者单位Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Wang, H-C,Jiang, W-P,Sima, Z-H,et al. Fibroblasts isolated from a keratocystic odontogenic tumor promote osteoclastogenesis in vitro via interaction with epithelial cells[J]. ORAL DISEASES,2015,21(2):170-177.
APA Wang, H-C,Jiang, W-P,Sima, Z-H,&Li, T-J.(2015).Fibroblasts isolated from a keratocystic odontogenic tumor promote osteoclastogenesis in vitro via interaction with epithelial cells.ORAL DISEASES,21(2),170-177.
MLA Wang, H-C,et al."Fibroblasts isolated from a keratocystic odontogenic tumor promote osteoclastogenesis in vitro via interaction with epithelial cells".ORAL DISEASES 21.2(2015):170-177.
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