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Aberrantly glycosylated serum IgA1 are closely associated with pathologic phenotypes of IgA nephropathy
Xu, LX1; Zhao, MH1
关键词Iga Nephropathy Glycosylation Pathology
刊名KIDNEY INTERNATIONAL
2005-07-01
68期:1页:167-172
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]HUMAN MESANGIAL CELLS ; MASS-SPECTROMETRY ; HINGE REGION ; O-GLYCOSYLATION ; BINDING ; MOLECULES ; GLYCANS ; CHAINS
英文摘要

Background. IgA nephropathy (IgAN) is the most common glomerulonephritis with various histologic and clinical phenotypes. The mechanisms underlying the pathogenesis of IgAN remained unclear. But now altered O-glycosylation of serum IgA1 observed in these patients was considered to be a key contributory factor. The aim of the current study is to investigate whether aberrantly glycosylated IgA1 was associated with pathologic phenotypes of IgAN.

Methods Sera from 107 patients with IgAN recently diagnosed were collected. Fifty patients were with mild mesangial proliferative IgAN, the others were with focal proliferative and sclerosing IgAN. Sera from 22 normal blood donors were used as normal controls. Biotinylated lectins were used in enzyme-linked immunosorbent assay (ELISA) to examine different glycans on IgA1 molecules. The alpha 2,6 sialic acid was detected by elderberry bark lectin (SNA), the exposure of terminal galactose (Gal) and N-acetylgalactosamine (GalNAc) were detected by arachis hypogaea [peanut agglutinin (PNA)] and vilsa villosa lectin (VVL), respectively. The serum IgA1 glycans levels corrected by serum IgA1 concentrations were compared between patients and controls.

Results. Reduced terminal alpha 2,6 sialic acid (1.16 +/- 0.21 vs. 0.98 +/- 0.31) (P= 0.008) and galactosylation (0.30 +/- 0.29 vs. 0.16 +/- 0.19) (P= 0.029) increased exposure of (GalNAc) (0.00 vs. 0.03) (P= 0.024) were demonstrated in serum IgA1 from patients with IgAN as compared with those in controls. More important, the exposures of 2,6 sialic acid and Gal were significantly decreased, especially in patients with focal proliferative and sclerosing IgAN compared with that in patients with mild mesangial proliferative IgAN (0.91 +/- 0.34 vs. 1.05 +/- 0.25) (P= 0.014) (0.108 +/- 0.137 vs. 0.221 +/- 0.219) (P= 0.018). However, no significant difference was found between patients with mild mesangial proliferative IgAN and normal controls (P > 0.05). The exposure of GalNAc of serum IgA1 from patients with focal proliferative and sclerosing IgAN was significantly higher than that of controls (P= 0.017), but had no statistical difference with that of patients with mild mesangial proliferative IgAN.

Conclusion. The desialylation and degalactosylation of IgA1 in sera of patients with IgAN were closely associated with pathologic phenotypes.

语种英语
WOS记录号WOS:000229636800015
引用统计
被引频次:62[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52259
专题北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ First Hosp, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ First Hosp, Inst Nephrol, Beijing 100034, Peoples R China
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GB/T 7714
Xu, LX,Zhao, MH. Aberrantly glycosylated serum IgA1 are closely associated with pathologic phenotypes of IgA nephropathy[J]. KIDNEY INTERNATIONAL,2005,68(1):167-172.
APA Xu, LX,&Zhao, MH.(2005).Aberrantly glycosylated serum IgA1 are closely associated with pathologic phenotypes of IgA nephropathy.KIDNEY INTERNATIONAL,68(1),167-172.
MLA Xu, LX,et al."Aberrantly glycosylated serum IgA1 are closely associated with pathologic phenotypes of IgA nephropathy".KIDNEY INTERNATIONAL 68.1(2005):167-172.
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