北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Toxic Effect of Methamphetamine on the Retina of CD1 Mice
作者: Lai, Hong2,3; Zhang, Cheng3; Wang, Lin3; Lai, Shenghan1; Zeng, Huiyang4,5; Tso, Mark O. M.3,6
关键词: Methamphetamine ; retinal damage ; CD1 mice ; neurotoxic effect
刊名: CURRENT EYE RESEARCH
发表日期: 2009
DOI: 10.1080/02713680903079641
卷: 34, 期:9, 页:785-790
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: FIBRILLARY ACIDIC PROTEIN ; RAT RETINA ; INDUCED NEUROTOXICITY ; MICROGLIAL ACTIVATION ; MOUSE-BRAIN ; DOPAMINE ; AMPHETAMINES ; STRIATUM ; MECHANISMS ; APOPTOSIS
英文摘要:

Purpose: To investigate whether systemic administration of methamphetamine (METH) induces retinal damage in CD1 mice. Materials and Methods: Eighteen male CD1 mice were randomly assigned to three groups, six mice per group: Group 1 receiving a single dose of 40 mg/kg METH, Group 2 receiving four doses of 10 mg/kg METH, and Group 3 (control) receiving 40 mg/kg 0.9% NaCl solution. METH and NaCl were administered by intraperitoneal injection. Immunostaining of glial fibrillary acidic protein (GFAP), S-100 for astrocytes and Muller cells, CD11b for microglia, and tyrosine hydroxylase (TH) and TUNEL labeling for apoptotic cell death were performed on the retinal sections on day 1 and day 7 post-exposure. Results: GFAP and S-100 immunoreactivity was observed in Group 1 mice. CD11b+ cells in Group 1mice showed more intensely stained shorter and thicker cellular processes than Groups 2 and 3, indicating activated microglia in the mice exposed to large-dose METH. No significant difference in TH level was seen among the three groups. TUNEL labeling did not reveal positive cells in the retinas of any of the 18 CD1 mice. Conclusions: A single large dose of METH induces an increase in short-term protein expression of GFAP and S-100 and in microglial activation. The results suggest that METH has a neurotoxic effect on CD1 mouse retina.

语种: 英语
所属项目编号: DA-15020
项目资助者: NIH/NIDA
WOS记录号: WOS:000271274900008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52262
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Bejing Ophthalmol & Visual Sci Key Lab, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
3.Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA
4.Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21205 USA
5.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA
6.Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing, Peoples R China

Recommended Citation:
Lai, Hong,Zhang, Cheng,Wang, Lin,et al. Toxic Effect of Methamphetamine on the Retina of CD1 Mice[J]. CURRENT EYE RESEARCH,2009,34(9):785-790.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Lai, Hong]'s Articles
[Zhang, Cheng]'s Articles
[Wang, Lin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Lai, Hong]‘s Articles
[Zhang, Cheng]‘s Articles
[Wang, Lin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace