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Simultaneous Detection and Identification of Aspergillus and Mucorales Species in Tissues Collected from Patients with Fungal Rhinosinusitis
Zhao, Zuotao1; Li, Lili2; Wan, Zhe1; Chen, Wei1; Liu, Honggang2; Li, Ruoyu1
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2011-04-01
DOI10.1128/JCM.02262-10
49期:4页:1501-1507
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
资助者First Hospital, Beijing University ; Ministry of Health ; Ministry of Science &amp ; Technology of China ; First Hospital, Beijing University ; Ministry of Health ; Ministry of Science &amp ; Technology of China
研究领域[WOS]Microbiology
关键词[WOS]LINE BLOT HYBRIDIZATION ; TRANSCRIBED SPACER REGION ; GENETIC IDENTIFICATION ; PCR ASSAY ; MUCORMYCOSIS ; DIAGNOSIS ; DNA ; POLYMORPHISM ; SPECIMENS ; DISEASE
英文摘要

Rapid detection and differentiation of Aspergillus and Mucorales species in fungal rhinosinusitis diagnosis are desirable, since the clinical management and prognosis associated with the two taxa are fundamentally different. We describe an assay based on a combination of broad-range PCR amplification and reverse line blot hybridization (PCR/RLB) to detect and differentiate the pathogens causing fungal rhinosinusitis, which include five Aspergillus species (A. fumigatus, A. flavus, A. niger, A. terreus, and A. nidulans) and seven Mucorales species (Mucor heimalis, Mucor racemosus, Mucor cercinelloidea, Rhizopus arrhizus, Rhizopus microsporus, Rhizomucor pusillus, and Absidia corymbifera). The assay was validated with 98 well-characterized clinical isolates and 41 clinical tissue specimens. PCR/RLB showed high sensitivity and specificity, with 100% correct identifications of 98 clinical isolates and no cross-hybridization between the species-specific probes. Results for five control isolates, Candida albicans, Fusarium solani, Scedosporium apiospermum, Penicillium marneffei, and Exophiala verrucosa, were negative as judged by PCR/RLB. The analytical sensitivity of PCR/RLB was found to be 1.8 x 10(-3) ng/mu l by 10-fold serial dilution of Aspergillus genomic DNA. The assay identified 35 of 41 (85.4%) clinical specimens, exhibiting a higher sensitivity than fungal culture (22 of 41; 53.7%) and direct sequencing (18 of 41; 43.9%). PCR/RLB similarly showed high specificity, with correct identification 16 of 18 specimens detected by internal transcribed spacer (ITS) sequencing and 16 of 22 detected by fungal culture, but it also has the additional advantage of being able to detect mixed infection in a single clinical specimen. The PCR/RLB assay thus provides a rapid and reliable option for laboratory diagnosis of fungal rhinosinusitis.

语种英语
所属项目编号200802026 ; 2008zx 10004-002
资助者First Hospital, Beijing University ; Ministry of Health ; Ministry of Science &amp ; Technology of China ; First Hospital, Beijing University ; Ministry of Health ; Ministry of Science &amp ; Technology of China
WOS记录号WOS:000289080600047
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52311
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
2.Capital Med Univ, Tongren Hosp, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Zuotao,Li, Lili,Wan, Zhe,et al. Simultaneous Detection and Identification of Aspergillus and Mucorales Species in Tissues Collected from Patients with Fungal Rhinosinusitis[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2011,49(4):1501-1507.
APA Zhao, Zuotao,Li, Lili,Wan, Zhe,Chen, Wei,Liu, Honggang,&Li, Ruoyu.(2011).Simultaneous Detection and Identification of Aspergillus and Mucorales Species in Tissues Collected from Patients with Fungal Rhinosinusitis.JOURNAL OF CLINICAL MICROBIOLOGY,49(4),1501-1507.
MLA Zhao, Zuotao,et al."Simultaneous Detection and Identification of Aspergillus and Mucorales Species in Tissues Collected from Patients with Fungal Rhinosinusitis".JOURNAL OF CLINICAL MICROBIOLOGY 49.4(2011):1501-1507.
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