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学科主题: 临床医学
题名:
Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection
作者: Zhang, X.1; Yan, Z.1; Zhang, J.1; Gong, L.1; Li, W.1; Cui, J.1; Liu, Y.1; Gao, Z.2; Li, J.2; Shen, L.1; Lu, Y.1
关键词: classifier ; gastric cancer ; microRNA ; recurrence
刊名: ANNALS OF ONCOLOGY
发表日期: 2011-10-01
DOI: 10.1093/annonc/mdq758
卷: 22, 期:10, 页:2257-2266
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: MICRORNA EXPRESSION ; CURATIVE RESECTION ; TOTAL RNA ; MICROARRAY ; SURGERY ; TARGETS ; SIGNATURE ; PROFILES ; PATTERNS ; LEUKEMIA
英文摘要:

Background: Recurrence is a major factor leading to treatment failure and death in gastric cancer (GC) patients following surgical resection. Importantly, the prediction of recurrence is critical in improving clinical outcomes. We isolated a group of microRNAs (miRNAs) and evaluated their usefulness as prognostic markers for the recurrence of GC.

Patients and methods: A total of 65 GC patients were selected for systematic analysis, 29 patients with recurrence and 36 patients without recurrence. Firstly, miRNAs microarray and bioinformatics methods were used to characterize classifiers from primary tumor samples (n = 8). Following, we validated these predictors both in frozen fresh and paraffin-embedded tissue samples (n = 57) using quantitative PCR.

Results: We have identified 17 differential miRNAs including 10 up-regulated and 7 down-regulated miRNAs in recurrence group. Using k-top scoring pairs (k-TSP) method, we further ascertained hsa-miR-375 and hsa-miR-142-5p as a classifier to recognize recurrence and nonrecurrence cases both in the training and test samples. Moreover, we validated this classifier in 34 frozen fresh tissues and 38 paraffin-embedded tissues with consistent sensitivity and specificity with training set; among them, 15 cases were matched. A high frequency recurrence and poor survival were observed in GC cases with high level of hsa-miR-375 and low level of hsa-miR-142-5p (P < 0.001). In addition, we evaluated that hsa-miR-375 and hsa-miR-142-5p were involved in regulating target genes in several oncogenic signal pathways, such as TP53, MAPK, Wnt and vascular endothelial growth factor.

Conclusion: Our results indicate that the combination of hsa-miR-375 and hsa-miR-142-5p as a predictor of disease progression has the potential to predict recurrence risk for GC patients.

语种: 英语
所属项目编号: 2006AA02A402 ; 2004CB518708 ; D0905001040631
项目资助者: National Bio-Tech 863 ; National Key Basic Research Program ; Beijing Municipal Science &amp ; Technology Commission
WOS记录号: WOS:000295168500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52312
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Mol Oncol Lab,Beijing Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.Beijing Univ Technol, Acad Elect Informat & Control Engn, Beijing, Peoples R China

Recommended Citation:
Zhang, X.,Yan, Z.,Zhang, J.,et al. Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection[J]. ANNALS OF ONCOLOGY,2011,22(10):2257-2266.
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