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学科主题: 临床医学
题名:
Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11;13)
作者: Zhuang, Xinjie1,2,3; Huang, Jin1,2,3; Jin, Xiaohu1,2,3; Yu, Yang1,2,3; Li, Junsheng1,2,3; Qiao, Jie1,2,3; Liu, Ping1,2,3
关键词: Infertility ; spermatogenesis ; Robertsonian translocation ; mouse
刊名: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
发表日期: 2014
卷: 7, 期:11, 页:7735-7743
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Pathology
研究领域[WOS]: Oncology ; Pathology
关键词[WOS]: PREIMPLANTATION GENETIC DIAGNOSIS ; HUMAN MALE-INFERTILITY ; IN-SITU HYBRIDIZATION ; PARENT-OF-ORIGIN ; MEIOTIC SEGREGATION ; SEMEN PARAMETERS ; SPERM ; MOUSE ; ANEUPLOIDY ; MEN
英文摘要:

Objective: To determine the diagnostic features of Robertsonian (Rob) translocation (11; 13) in mice and the mechanisms underlying the effect on spermatogenesis and reproductive decline. Methods: A Rob translocation (11; 13) mouse model was established by cross-breeding, and confirmed by chromosome analysis. Chromosome aberrations and translocation patterns were identified in mice with Rob translocation (11; 13) by fluorescence in situ hybridization (FISH). Spermatogenic disorders were investigated at different stages of spermatogenesis. Immunofluorescent analysis was performed on sections of testis and epididymis specimens during spermatogenic meiosis. The weight of the testes and reproductive decline were recorded. Results: The crossed Rob translocation (11; 13) mouse has 39 chromosomes, including a fusion chromosome (included chromosomes 11 and 13) using dual color FISH. There was no difference in the distribution pattern of SYCP3 and gamma H2AX in spermatocytes between Rob translocation and wild-type mice; however, round haploid spermatids presented characteristic morphologic changes of apoptosis and the number of haploid spermatids was decreased. Furthermore, the immature germ cells were released into the epididymis and the number of mature sperm was reduced. Conclusions: Chromosome aberrations and spermatogenic disorders may result from apoptosis of round haploid spermatids and a reduced number of mature sperm in Rob translocation (11; 13) mice. Abnormal sperm and reduced number of sperm may be one of the main reasons for reproductive decline and male infertility in Rob translocation (11; 13) mice.

语种: 英语
所属项目编号: 81070534 ; 81200466
项目资助者: National Natural Science Foundation of China ; National Natural Science Foundation of China for Young Scholars
WOS记录号: WOS:000348345200044
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52322
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China

Recommended Citation:
Zhuang, Xinjie,Huang, Jin,Jin, Xiaohu,et al. Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11;13)[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(11):7735-7743.
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