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学科主题临床医学
Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11;13)
Zhuang, Xinjie1,2,3; Huang, Jin1,2,3; Jin, Xiaohu1,2,3; Yu, Yang1,2,3; Li, Junsheng1,2,3; Qiao, Jie1,2,3; Liu, Ping1,2,3
关键词Infertility Spermatogenesis Robertsonian Translocation Mouse
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2014
7期:11页:7735-7743
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pathology
研究领域[WOS]Oncology ; Pathology
关键词[WOS]PREIMPLANTATION GENETIC DIAGNOSIS ; HUMAN MALE-INFERTILITY ; IN-SITU HYBRIDIZATION ; PARENT-OF-ORIGIN ; MEIOTIC SEGREGATION ; SEMEN PARAMETERS ; SPERM ; MOUSE ; ANEUPLOIDY ; MEN
英文摘要

Objective: To determine the diagnostic features of Robertsonian (Rob) translocation (11; 13) in mice and the mechanisms underlying the effect on spermatogenesis and reproductive decline. Methods: A Rob translocation (11; 13) mouse model was established by cross-breeding, and confirmed by chromosome analysis. Chromosome aberrations and translocation patterns were identified in mice with Rob translocation (11; 13) by fluorescence in situ hybridization (FISH). Spermatogenic disorders were investigated at different stages of spermatogenesis. Immunofluorescent analysis was performed on sections of testis and epididymis specimens during spermatogenic meiosis. The weight of the testes and reproductive decline were recorded. Results: The crossed Rob translocation (11; 13) mouse has 39 chromosomes, including a fusion chromosome (included chromosomes 11 and 13) using dual color FISH. There was no difference in the distribution pattern of SYCP3 and gamma H2AX in spermatocytes between Rob translocation and wild-type mice; however, round haploid spermatids presented characteristic morphologic changes of apoptosis and the number of haploid spermatids was decreased. Furthermore, the immature germ cells were released into the epididymis and the number of mature sperm was reduced. Conclusions: Chromosome aberrations and spermatogenic disorders may result from apoptosis of round haploid spermatids and a reduced number of mature sperm in Rob translocation (11; 13) mice. Abnormal sperm and reduced number of sperm may be one of the main reasons for reproductive decline and male infertility in Rob translocation (11; 13) mice.

语种英语
WOS记录号WOS:000348345200044
项目编号81070534 ; 81200466
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China for Young Scholars
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52322
专题北京大学第三临床医学院_妇产科
北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_生殖医学中心
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhuang, Xinjie,Huang, Jin,Jin, Xiaohu,et al. Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11;13)[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(11):7735-7743.
APA Zhuang, Xinjie.,Huang, Jin.,Jin, Xiaohu.,Yu, Yang.,Li, Junsheng.,...&Liu, Ping.(2014).Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11;13).INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,7(11),7735-7743.
MLA Zhuang, Xinjie,et al."Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11;13)".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 7.11(2014):7735-7743.
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