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学科主题基础医学
Determination of pharmacokinetics of 8-chloroadenosine and its two major metabolites in dogs by high-performance liquid chromatography
Wang, XY1; Zhang, WQ1; Zou, AQ1; Lou, YQ1
关键词8-chloroadenosine 8-chloroadenine 8-chloroinosine
刊名JOURNAL OF CHROMATOGRAPHY B
2000-09-15
746期:2页:319-323
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
英文摘要

High-performance liquid chromatography was used to measure concentration of 8-chloroadenosine (8-Cl-A) and its two major metabolites 8-chloroadenine (8-Cl-Ad) and 8-chloroinosine (8-Cl-I), and their pharmacokinetics in dogs. 8-Cl-A and its metabolites in serum were treated by deproteinization with acetonitrile, then organic impurities were extracted with dichloromethane, followed by centrifuged and direct injection of the supernatant into the liquid chromatograph. After intravenous injection of 8-Cl-A (30 mg/kg), the parent drug and 8-Cl-I were not detected, but the other metabolite, 8-Cl-Ad, was found at a high concentration for 240 min in dog serum. The main pharmacokinetic parameters of 8-Cl-Ad, t(1/2 beta) and AUC, were 69.30 min and 580 mu g min/ml. Our finding indicates that in dogs 8-Cl-A is rapidly metabolized and forms its major metabolites, 8-Cl-Ad and 8-Cl-I. 8-Cl-Ad appeared in many tissues, but 8-Cl-A and 8-Cl-I did not. The concentration of 8-Cl-Ad in dog tissues was highest in the liver and spleen, intermediate in the kidney, intestine, and lowest in the bone marrow, heart, and lungs. However, it was not detected in some liposoluble tissues such as the testes, brain, or uterus. Our study provides useful information for clinical experiment. (C) 2000 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000089429300024
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52326
专题北京大学基础医学院_药理学系
北京大学药学院
作者单位1.Beijing Med Univ, Coll Pharm, Beijing 100083, Peoples R China
2.Beijing Med Univ, Dept Pharmacol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Wang, XY,Zhang, WQ,Zou, AQ,et al. Determination of pharmacokinetics of 8-chloroadenosine and its two major metabolites in dogs by high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B,2000,746(2):319-323.
APA Wang, XY,Zhang, WQ,Zou, AQ,&Lou, YQ.(2000).Determination of pharmacokinetics of 8-chloroadenosine and its two major metabolites in dogs by high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY B,746(2),319-323.
MLA Wang, XY,et al."Determination of pharmacokinetics of 8-chloroadenosine and its two major metabolites in dogs by high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY B 746.2(2000):319-323.
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