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学科主题: 基础医学
题名:
Determination of pharmacokinetics of 8-chloroadenosine and its two major metabolites in dogs by high-performance liquid chromatography
作者: Wang, XY1; Zhang, WQ1; Zou, AQ1; Lou, YQ1
关键词: 8-chloroadenosine ; 8-chloroadenine ; 8-chloroinosine
刊名: JOURNAL OF CHROMATOGRAPHY B
发表日期: 2000-09-15
卷: 746, 期:2, 页:319-323
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
英文摘要:

High-performance liquid chromatography was used to measure concentration of 8-chloroadenosine (8-Cl-A) and its two major metabolites 8-chloroadenine (8-Cl-Ad) and 8-chloroinosine (8-Cl-I), and their pharmacokinetics in dogs. 8-Cl-A and its metabolites in serum were treated by deproteinization with acetonitrile, then organic impurities were extracted with dichloromethane, followed by centrifuged and direct injection of the supernatant into the liquid chromatograph. After intravenous injection of 8-Cl-A (30 mg/kg), the parent drug and 8-Cl-I were not detected, but the other metabolite, 8-Cl-Ad, was found at a high concentration for 240 min in dog serum. The main pharmacokinetic parameters of 8-Cl-Ad, t(1/2 beta) and AUC, were 69.30 min and 580 mu g min/ml. Our finding indicates that in dogs 8-Cl-A is rapidly metabolized and forms its major metabolites, 8-Cl-Ad and 8-Cl-I. 8-Cl-Ad appeared in many tissues, but 8-Cl-A and 8-Cl-I did not. The concentration of 8-Cl-Ad in dog tissues was highest in the liver and spleen, intermediate in the kidney, intestine, and lowest in the bone marrow, heart, and lungs. However, it was not detected in some liposoluble tissues such as the testes, brain, or uterus. Our study provides useful information for clinical experiment. (C) 2000 Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000089429300024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52326
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Beijing Med Univ, Coll Pharm, Beijing 100083, Peoples R China
2.Beijing Med Univ, Dept Pharmacol, Beijing 100083, Peoples R China

Recommended Citation:
Wang, XY,Zhang, WQ,Zou, AQ,et al. Determination of pharmacokinetics of 8-chloroadenosine and its two major metabolites in dogs by high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B,2000,746(2):319-323.
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